| 货号 | MAB9101 |
| 别名 | EC 3.4.24; EC 3.4.24.22; matrix metallopeptidase 10 (stromelysin 2); matrix metalloprotease 10; matrix metalloproteinase 10 (stromelysin 2); Matrix metalloproteinase-10; MMP-10; SL-2; STMY2stromelysin-2; Stromelysin 2; transin 2; transin-2 | 全称 | Matrix Metalloproteinase 10 |
| 反应种属 | Human |
| 应用 | Western Blot,Immunohistochemistry,Immunoprecipitation,Neutralization |
| 目标/特异性 | Detects the pro and active forms of human MMP-10 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human MMP-1, -2, -3, -7, -8, -9, -12, or -13 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 8-25 µg/mL Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human MMP‑10 (1 µg/mL, Catalog # 910‑MP) cleavage of the fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (10 µM, Catalog # ES002). The Neutralization Dose (ND50) is typically 1 µg/mL. |
| 来源 | Monoclonal Mouse IgG2B Clone # 117239 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4319 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. TRE17/USP6 oncogene translocated in aneurysmal bone cyst induces matrix metalloproteinase production via activation of NF-kappaB. | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human MMP-10 Tyr18-Cys476 Accession # P09238 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-10 (stromelysin 2) degrades a broad range of substrates including gelatin, collagen types III, IV and V, fibronectin, aggrecan, and pig cartilage proteoglycan. MMP-10 can activate other MMPs such as MMP-1 and MMP-8. MMP-10 is expressed in keratinocytes, T cells, menstrual endometrium, and a few tumor samples. Structurally, MMP-10 may be divided into four distinct domains: a pro-domain which is cleaved upon activation, a catalytic domain containing the zinc binding site; a short linker region, and a carboxyl terminal hemopexin-like domain. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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