| 货号 | D044-3 |
| 描述 | For ELISA the Antibody Pairs information：Capture antibody：D044-3；Detection antibody：D045-6; and protein: B001-5 |
| 别名 | Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18/IL-1F4; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18 | 全称 | Interleukin 18 |
| 反应种属 | Human |
| 应用 | Immunoprecipitation,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization |
| 目标/特异性 | This antibody reacts with human IL-18 on Immunoprecipitation. |
| 使用方法 | Immunoprecipitation: 5 μg/0.5 μg recombinant human IL-18 ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.5-2.0 µg/mL ELISA Standard : Neutralization: Induction of IFN-gamma by KG-1 cell (Human myelomonocyte: ATCC CCL246) in response to the 40 ng/mL recombinant Human IL-18 was neutralized by this antibody. |
| 来源 | Monoclonal Mouse IgG1 Clone # 125-2H |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Influenza Vaccination Generates Cytokine-Induced Memory-like NK Cells: Impact of Human Cytomegalovirus Infection | |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | Human IL-18/IL-1F4 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 背景 | Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells. |
| 运输条件 | Dry Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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