| 货号 | MAB8271 |
| 别名 | apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl-2 | 全称 | B Cell Lymphoma/Leukemia 2 |
| 反应种属 | Human |
| 应用 | Neutralization |
| 目标/特异性 | Detects human Bcl-2 Minus C-Terminus. Neutralizes recombinant human (rh) Bcl-2 bioactivity but not rhBcl-w or rhBcl-x bioactivity. |
| 使用方法 | Neutralization: Measured by its ability to neutralize Recombinant Human Bcl-2 (Catalog # 827-BC) inhibition of caspase-8-cleaved Recombinant Human BID (Catalog # 882-B8) induced release of cytochrome c from isolated mouse liver mitochondria using the Rat/Mouse Cytochrome c Quantikine ELISA Kit (Catalog # MCTC0). |
| 来源 | Monoclonal Mouse IgG1 Clone # 118712 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 596 (Human); 12043 (Mouse); 24224 (Rat) |
| 纯化方式 | Protein A or G purified from hybridoma culture supernatant |
| 免疫原 | E. coli-derived recombinant human Bcl-2 minus the carboxyl-terminal mitochondrial targeting sequence |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | Bcl-2 is a member of a family of proteins that regulates outer mitochondrial membrane permeability (1, 2). Bcl-2 is an anti-apoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-2 is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-2 contains a carboxyl-terminal mitochondria targeting sequence. Recombinant Bcl-2, missing the mitochondrial targeting sequence, maintains its ability to neutralize pro-apoptotic Bcl-2 family members. Neutralization by Bcl-2 appears to be through binding the BH3 region of pro-apoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
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| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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