| 货号 | MAB327-SP |
| 别名 | C-C motif chemokine 13; chemokine (C-C motif) ligand 13; CKb10; MCP4; MCP-4; MCP-4Monocyte chemoattractant protein 4; MGC17134; NCC-1Monocyte chemotactic protein 4; NCC1Small-inducible cytokine A13; new CC chemokine 1; SCYA13CK-beta-10; SCYL1; small inducible cytokine subfamily A (Cys-Cys), member 13 |
| 反应种属 | Human |
| 应用 | CyTOF-ready,Intracellular Staining by Flow Cytometry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization |
| 目标/特异性 | Detects human CCL13/MCP‑4 in ELISAs. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) CCL2 is observed and no significant cross-reactivity or interference with rhCCL5,7, 8, 11, 24, recombinant mouse CCL2, 5, 7, 11 or 12 is observed. |
| 使用方法 | Intracellular Staining by Flow Cytometry: 2.5 µg/106cells ELISA Capture (Matched Antibody Pair): 2-8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize CCL13/MCP‑4-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 10-30 µg/mL in the presence of 2 µg/mL Recombinant Human CCL13/MCP‑4. |
| 来源 | Monoclonal Mouse IgG1 Clone # 73506 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6357 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines. | |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | E. coli-derived recombinant human CCL13/MCP-4 Gln24-Thr98 Accession # Q99616.1 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | CCL13, also known as Monocyte Chemoattractant Protein-4 (MCP-4), is a CC chemokine that acts as a chemoattractant for monocytes, eosinophils and T cells and as an activator of basophils. Human CCL13 cDNA encodes a 98 amino acid residue precursor protein with a 23 amino acid residue hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature CCL13. Mature CCL13 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins. Human CCL13 is most homologous to MCP-1, 3 and Eotaxin, exhibiting approximately 65-66% amino acid sequence identity. CCL13 mRNA is expressed by a number of activated cell types, including endothelial cells, macrophages, bronchial epithelium and type II alveolar cells, and perhaps lymphocytes. The bioactivities of CCL13 are mediated by the CC chemokine receptors CCR-2 and CCR-3. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Chemotaxis Induced by CCL13/MCP‑4 and Neutralization by Human CCL13/ MCP‑4 Antibody. Recombinant Human CCL13/ MCP‑4 (Catalog # 327‑P4) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL13/MCP‑4 (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti‑Human CCL13/ MCP‑4 Monoclonal Antibody (Catalog # MAB327). The ND50 is typically 10‑30 µg/mL. |
