| 货号 | MAB371-SP |
| 别名 | CCL23; MPIF-1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Intracellular Staining by Flow Cytometry(0.25 µg/106cells) |
| 目标/特异性 | Detects human CCL23/MPIF‑1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Intracellular Staining by Flow Cytometry: 0.25 µg/106cells Neutralization: Measured by its ability to neutralize CCL23/MPIF‑1-induced chemotaxis in the THP‑1 human acute monocytic leukemia cell line. Howell, D. N. et al., 1985, J. Immunol. 134:971. The Neutralization Dose (ND50) is typically 2‑6 µg/mL in the presence of 1 µg/mL Recombinant Human CCL23/MPIF‑1. |
| 来源 | Monoclonal Mouse IgG1 Clone # 69512 |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6368 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. CCL23 expression is induced by IL-4 in a STAT6-dependent fashion. | |
| 纯化方式 | Protein A or G purified from ascites |
| 免疫原 | E. coli-derived recombinant human CCL23/MPIF-1 Arg22-Asn120 Accession # P55773 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | CCL23, also known as Myeloid Progenitor Inhibitory Factor-1 (MPIF-1),is a CC chemokine that is produced by some myeloid cell lines andmonocyte-derived dendritic cells. It binds CCR1 and inhibits thedifferentiation of progenitor cells that give rise to granulocytes andmacrophages. CCL23 is also chemotactic for resting T cells,neutrophils, monocytes and dendritic cells. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Chemotaxis Induced by CCL23/MPIF‑1 and Neutralization by Human CCL23/MPIF‑1 Antibody. Recombinant Human CCL23/MPIF‑1 (Catalog # 371-MP) chemoattracts the THP‑1 human acute monocytic leukemia cell line in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL23/MPIF‑1 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL23/MPIF‑1 Monoclonal Antibody (Catalog # MAB371). The ND50 is typically 2-6 µg/mL. |
