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货号： bs-19247R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-19247R
- 英文名称
- NINL
- 中文名称
- Ninein样蛋白抗体
- 别 名
- 4930519N13Rik; dJ691N24.1; Gm1004,; Gm1634; mKIAA0980; Ninein like; Ninein-like protein; NINL; NINL_HUMAN; Nlp; RGD1306152; RP23-193L22.3; RP4-691N24.1.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物 细胞周期蛋白 细胞分化
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 156kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human NINL:401-500/1382
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20癈. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 癈.
- PubMed
- PubMed
- 产品介绍
- background:
NINL is a 1,382 amino acid protein that localizes to both the cytoplasm and the centrosome and contains four EF-hand domains. Interacting with ?Tubulin, NLP is involved in microtubule organization in interphase cells and, when overexpressed, causes lysosomal dispersion and interferes with mitotic spindle assembly. NLP is subject to post-translational phosphorylation by Plk, an event which disrupts the association of NLP with centrosomes.
Function:
Involved in the microtubule organization in interphase cells. Overexpression induces the fragmentation of the Golgi, and causes lysosomes to disperse toward the cell periphery; it also interferes with mitotic spindle assembly. May play a role in ovarian carcinogenesis
Subunit:
nteracts with gamma-tubulin and TUBGCP4. Interacts with anaphase promoting complex/cyclosome (APC/C). Interacts with CDC20 and FZR1. Isoform 2 interacts with LCA5 and USH2A.
Subcellular Location:
Cytoplasm > cytoskeleton > centrosome. Cytoplasm. In interphase cells, NINL is transported to the centrosomes by the dynein-dynactin motor complex. During centrosome maturation, PLK1 directly phosphorylates NINL resulting in its release into the cytoplasm.
Tissue Specificity:
Expressed in KYSE150 esophageal carcinoma, HeLa cervical carcinoma and U2OS osteosarcoma cells. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase (at protein level). Expressed in fetal heart, skeletal muscle, liver, lung and cochlea, and in adult brain, testis, kidney and retina.
Post-translational modifications:
Phosphorylated by PLK1 which disrupts its centrosome association and interaction with gamma-tubulin.
Ubiquitinated by the APC/C complex leading to its degradation.
Similarity:
Contains 4 EF-hand domains.
SWISS:
Q9Y2I6
Gene ID:
22981
Database links:Entrez Gene: 477006Dog
Entrez Gene: 100057245Horse
Entrez Gene: 22981Human
Entrez Gene: 78177Mouse
Entrez Gene: 100519522Pig
Entrez Gene: 311529Rat
Omim: 609580Human
SwissProt: Q9Y2I6 Human
SwissProt: Q6ZQ12Mouse
Unigene: 631508Human
Unigene: 120911Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.