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货号： bs-11367R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-11367R
- 英文名称
- SYNPR/Synaptoporin
- 中文名称
- 突触囊泡蛋白抗体
- 别 名
- SPO; Synaptophysin 2; Synaptoporin; Synpr; SYNPR_HUMAN; DKFZp686G0883; MGC26651.
- Specific References (1) | bs-11367R has been referenced in 1 publications.[IF=7.26] Zhang, Chi, et al. "The potential use of H102 peptide-loaded dual-functional nanoparticles in the treatment of Alzheimer’s disease." Journal of Controlled Release (2014). WB ; Mouse.PubMed:25102404
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物 神经生物学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 29kDa
- 细胞定位
- 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Synaptoporin:31-130/265
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Synaptic vesicle recycling involves numerous proteins that contribute to the formation and trafficking of the SNARE complexes throughout the cell. Synaptoporin, also designated synaptophysin 2, is an integral membrane protein of small synaptic vesicles that belongs to the synaptophysin/synaptobrevin family. Synaptoporin is highly homologus to Synaptophysin 1 and both Synaptoporin and synaptophysin 1 contain four transmembrane domains and a short cytoplasmic tail. The Synaptoporin protein also contains one MARVEL domain, a membrane-associating domain found in lipid-associating proteins, and displays calcium-binding activity which may be localized to its cytoplasmic tail. Syntaphilin, synaptophysin and Synaptoporin regulate the formation of the vesicles by competing with components of the SNARE complexes to respectively inhibit either the assembly or the secretion of the synaptic vesicles.
Function:
Intrinsic membrane protein of small synaptic vesicles. Probable vesicular channel protein.
Subcellular Location:
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse >synaptosome.
Similarity:
Belongs to the synaptophysin/synaptobrevin family.
Contains 1 MARVEL domain.
SWISS:
Q8TBG9
Gene ID:
132204
Database links:Entrez Gene: 132204 Human
Entrez Gene: 72003 Mouse
Entrez Gene: 66030 Rat
SwissProt: Q8TBG9 Human
SwissProt: Q8BGN8 Mouse
SwissProt: P22831 Rat
Unigene: 648668 Human
Unigene: 317515 Mouse
Unigene: 211986 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Bulbus olfactorius (Rat) Lysate at 40 ug
Primary: Anti-Synaptoporin (bs-11367R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 28 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SYNPR) Polyclonal Antibody, Unconjugated (bs-11367R) at 1:800 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control(blue): RSC96(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-SYNPR/Synaptoporin antibody(bs-11367R), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.