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货号: bs-1396R 基本售价: 780.0 元 规格: 50ul
- 规格:50ul
- 价格:780.00元
- 规格:100ul
- 价格:1380.00元
- 规格:200ul
- 价格:2200.00元
产品信息
- 产品编号
- bs-1396R
- 英文名称
- CEBP Beta
- 中文名称
- 转录调节因子C/EBP β抗体
- 别 名
- CEBP-beta; CLEBP beta; CCAAT Enhancer Binding Protein beta; AGP/EBP; C EBP beta; C/EBP related protein 2; CEBPB; CRP2; IL 6DBP; IL6DBP; Interleukin 6 dependent binding protein; LAP; Liver enriched transcriptional activator; NF IL6; NFIL6; Nuclear factor NF IL6; SF B; SFB; Silencer factor B; TCF5; Transcription factor 5; C/EBP beta; CCAAT/enhancer-binding protein beta; CEBPB_HUMAN; Liver activator protein; Liver enriched transcriptional activator; Nuclear factor NF IL6; Nuclear factor NF-IL6; TCF-5.
- Specific References (1) | bs-1396R has been referenced in 1 publications.[IF=5.62] He, Ting, et al. "Tumor cell-secreted angiogenin induces angiogenic activity of endothelial cells by suppressing miR-542-3p." Cancer Letters (2015). WB ; Human.PubMed:26272182
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 染色质和核信号 转录调节因子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Pig, Cow, Rabbit, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1µg/Test IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 36kDa
- 细胞定位
- 细胞核
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human CEBP Beta:251-345/345
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. The encoded protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, the encoded protein can bind the promoter and upstream element and stimulate the expression of the collagen type I gene. [provided by RefSeq].
Function:
Involved in interleukin-6 signal transduction, including the transcriptional activation of acute-phase genes. Functions in brown adipose tissue (BAT) differentiation.
Subunit:
Binds DNA as a dimer and can form stable heterodimers with C/EBP alpha and gamma. Interacts with TRIM28 and PTGES2. Interacts with PRDM16. Interacts with CCDC85B.
Subcellular Location:
Nucleus.
Post-translational modifications:
Sumoylated by polymeric chains of SUMO2 or SUMO3.
Similarity:
Belongs to the bZIP family. C/EBP subfamily.
Contains 1 bZIP domain.
SWISS:
P17676
Gene ID:
1051
Database links:Entrez Gene: 1051 Human
Entrez Gene: 12608 Mouse
Entrez Gene: 24253 Rat
Omim: 189965 Human
SwissProt: P17676 Human
SwissProt: P28033 Mouse
SwissProt: P21272 Rat
Unigene: 517106 Human
Unigene: 719041 Human
Unigene: 720603 Human
Unigene: 439656 Mouse
Unigene: 6479 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
转录调节因子(Transcriptin Regulators)
- 产品图片
- Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-CEBP Beta (bs-1396R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 41 kDSample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-CEBP Beta (bs-1396R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kDSample:
MG63(Human) Cell Lysate at 40 ug
Primary: Anti-CEBP Beta (bs-1396R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kDSample:
HepG2(Human) Cell Lysate at 40 ug
Primary: Anti-CEBP Beta (bs-1396R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kDParaformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CEBP Beta) Polyclonal Antibody, Unconjugated (bs-1396R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Blank control (blue line): Hela
Primary Antibody (green line): Rabbit Anti-CEBP Beta antibody (bs-1396R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.