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货号： bs-8589R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-8589R
- 英文名称
- Transglutaminase 2
- 中文名称
- 谷氨酰胺转胺酶2抗体
- 别 名
- TG 2; TG(C); TG2; TGase C; TGase H; TGase-2; TGase2; TgaseII; ALPHA SUBUNIT; C polypeptide; G alpha h; G protein alpha subunit Gh class; G[a]h; Gh CLASS G ALPHA h; GNAH; GNAH G PROTEIN; Guanine nucleotide binding protein H polypeptide; H POLYPEPTIDE; Protein glutamine gamma glutamyltransferase 2; Protein glutamine gamma glutamyltransferase; Protein-glutamine gamma-glutamyltransferase 2; TGC; TGC GUANINE NUCLEOTIDE BINDING PROTEIN; TGM 2; TGM2; TGM2_HUMAN; Tissue transglutaminase; Tissue type transglutaminase; Transglutaminase 2; Transglutaminase 2 C polypeptide; Transglutaminase C; Transglutaminase H; Transglutaminase-2; tTG; tTGas.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 信号转导 转录调节因子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 77kDa
- 细胞定位
- 细胞浆 细胞膜 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Transglutaminase 2:351-450/687
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Terminally differentiating mammalian epidermal cells acquire an insoluble, 10 to 20 nm thick protein deposit on the intracellular surface of the plasma membrane known as the cross-linked cell envelope (CE). The CE is a component of the epidermis that is generated through formation of disulfide bonds and g-glutamyl-lysine isodipeptide bonds, which are formed by the action of transglutaminases (TGases). TGases are intercellularly localizing, Ca2+-dependent enzymes that catalyze the formation of isopeptide bonds by transferring an amine on to glutaminyl residues, thereby cross-linking glutamine residues and lysine residues in substrate proteins. TGases influence numerous biological processes, including blood coagulation, epidermal differentiation, seminal fluid coagulation, fertilization, cell differentiation and apoptosis. Human keratinocyte transglutaminase (TGase1) is a membrane associated, 817 amino acid protein. Human tissue transglutaminase (TGase2) is an endothelial cell specific, 687 amino acid protein.
Function:
Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
Subunit:
Monomer.
Similarity:
Belongs to the transglutaminase superfamily. Transglutaminase family.
SWISS:
P21980
Gene ID:
7052
Database links:Entrez Gene: 7052Human
Entrez Gene: 21817Mouse
Omim: 190196Human
SwissProt: P21980Human
SwissProt: P21981Mouse
Unigene: 517033Human
Unigene: 330731Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Uterus (Mouse) Lysate at 40 ug
Primary: Anti-Transglutaminase 2 (bs-8589R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 77 kD
Observed band size: 74 kD
Paraformaldehyde-fixed, paraffin embedded (human Pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TGM2) Polyclonal Antibody, Unconjugated (bs-8589R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TGM2) Polyclonal Antibody, Unconjugated (bs-8589R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.