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货号： bs-8551R 基本售价： 1580.0 元 规格： 100ul
产品信息
- 产品编号
- bs-8551R
- 英文名称
- 3-Nitrotyrosine
- 中文名称
- 硝基酪氨酸/硝基化酪氨酸抗体
- 别 名
- NO tyrosine; nTyr; Nitrotyrosine; nitro-Tyrosine; nitro Tyrosine. 酪氨酸硝基化；
- Specific References (4) | bs-8551R has been referenced in 4 publications.[IF=2.43] Zhang, Jing-Yao, et al. "Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice." World Journal of Gastroenterology 21.14 (2015): 4195-4209. IHC-P ; Mouse.PubMed:25892869[IF=4.38] Chen, Yu-Lin, and Wai-Ming Kan. "Down-regulation of superoxide dismutase 1 by PMA is involved in cell fate determination and mediated via protein kinase D2 in myeloid leukemia cells." Biochimica et Biophysica Acta (BBA)-Molecular Cell Research (2015). Human.PubMed:26241492[IF=3.40] Wang, Lin-Feng, et al. "Tert-butylhydroquinone ameliorates doxorubicin-induced cardiotoxicity by activating Nrf2 and inducing the expression of its target genes." American Journal of Translational Research 7.10 (2015): 1724-1735. IHC-P ; Mouse.PubMed:26692920[IF=5.78] Li, Dawei, et al. "Hepatic Hypoxia-Inducible Factors Inhibit PPARα Expression To Exacerbate Acetaminophen induced Oxidative Stress And Hepatotoxicity." Free Radical Biology and Medicine (2017). IHC-P ; Mouse.PubMed:28583670
- 规格价格
- 100ul/1580元购买 大包装/询价
- 说 明 书
- 100ul
- 产品类型
- 药物与化合物抗体
- 研究领域
- 肿瘤 细胞生物 信号转导 Alzheimers
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Rat, Nitrotyrosine
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated to Nitrotyrosine:
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Nitrotyrosine is a marker for inflammation and nitric oxide (NO) production and is formed in the presence of the active metabolite NO. Because nitrotyrosine is a stable product of multiple pathways, such as the formation of peroxynitrite, its plasma concentration may be a useful determinant of NO-dependent damage in vivo. Nitrotyrosine has been detected in inflammatory processes such as septic shock, rheumatoid arthritis, celiac disease, atherosclerotic plaques and chronic renal failure.
Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A, SP-A, to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis.
Subcellular Location:
Cytoplasm.
SWISS:
N/A
CAS:
621-44-3
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample: 8551PB(3-Nitrotyrosine-BSA conjugate Protein )
Primary:Anti-3-Nitrotyrosine (bs-8551R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nitro tyrosine Polyclonal Antibody, Unconjugated(bs-8551R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice..
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).