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货号： bs-9294R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-9294R
- 英文名称
- BTBD1
- 中文名称
- BTB/POZ结构域蛋白1（丙型肝炎病毒的NS5A反转录蛋白8）抗体
- 别 名
- BTB (POZ) domain containing 1; BTB domain containing 1; BTB/POZ domain containing protein 1; BTBD 1; BTBD1; C15orf1; HCV NS5A transactivated protein 8; Hepatitis C virus NS5A transactivated protein 8; NS5ATP8; BTBD1_HUMAN.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物 细胞周期蛋白 转录调节因子 细菌及病毒 细胞分化
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 53kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human BTBD1:101-200/482
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
BTBD1 contains a proline-rich region and a BTB/POZ domain which are typically involved in protein-protein interactions. The C-terminus of BTBD1 binds topoisomerase I and BTBD1 also interacts with TRIM5 isoform Delta. BTBD1 localizes to cytoplasmic bodies and is essential for myogenic differentiation.
Function:
Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
Subunit:
Interacts with topoisomerase 1 and with TRIM5 isoform Delta. Interacts with CUL3.
Subcellular Location:
Cytoplasm, P-body. Cytoplasmic bodies.
Tissue Specificity:
Ubiquitous; higher levels in heart and skeletal muscle.
Similarity:
Contains 1 BTB (POZ) domain.
SWISS:
Q9BSF8
Gene ID:
84280
Database links:Entrez Gene: 84280 Human
Entrez Gene: 68815 Mouse
Entrez Gene: 308890 Rat
SwissProt: Q9BSF8 Human
SwissProt: Q80X66 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample: Testis (Mouse) Lysate at 40 ug
Primary: Anti-BTBD1 (bs-9294R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-BTBD1 (bs-9294R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BTBD1) Polyclonal Antibody, Unconjugated (bs-9294R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.