产品中心
当前位置：首页>产品中心Anti-Phospho-CRMP2 (Thr514+Ser518)
货号： bs-8284R 基本售价： 1580.0 元 规格： 100ul
产品信息
- 产品编号
- bs-8284R
- 英文名称
- Phospho-CRMP2 (Thr514+Ser518)
- 中文名称
- 磷酸化二氢嘧啶酶样2抗体
- 别 名
- CRMP2 (phospho T514 + S518); CRMP2 (phospho T514+S518); CRMP-2(phospho Thr514+S518); p-CRMP2(Thr514+S518); CRMP2/Dpysl2(DRP 2; Collapsin response mediator protein 2; Collapsin response mediator protein hCRMP 2; CRAM; CRMP 2; DHPRP 2; DHPRP2; Dihydr pyrimidinase 2; Dihydropyrimidinase 2; Dihydropyrimidinase like 2; Dihydropyrimidinase like 2 long form;Dihydropyrimidinase related protein 2; DPYL 2; DPYL2; DPYSL 2; DPYSL2; DRP 2; DRP2; Musunc 33; Musunc33; N2A3; TOAD 64; TOAD64; ULIP 2 protein; Ulip2.
- 规格价格
- 100ul/1580元购买 大包装/询价
- 说 明 书
- 100ul
- 产品类型
- 磷酸化抗体
- 研究领域
- 细胞生物 免疫学 神经生物学 生长因子和激素
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Sheep,
- 产品应用
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 62kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthesised phosphopeptide derived from human CRMP2 around the phosphorylation site of Thr514+Ser518:TV(p-T)PAS(p-S)AK
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Collapsin response mediator proteins (CRMPs) are cytosolic phosphoproteins involved in neuronal differentiation and axonal guidance. CRMP2 was previously shown to mediate the repulsive effect of Sema3A on axons and to participate in axonal specification. The CRMPs appear to play a complex role in axon growth as well as microtubule dynamics and axon induction. CRMPs localize to the lamellipodia and filopodia of axonal growth cones, suggesting a role in axon guidance. Moreover, CRMP2 is upregulated after axotomy, and appears to increase the formation of axon-type processes from hippocampal neurons. CRMP2 has been reported to bind tubulin dimers directly and modulate microtubule assembly. CRMPs have also been implicated in the pathogenesis of a paraneoplastic neurologic syndrome. Interaction studies have implicated phospholipase D2 (PLD2), the cytosolic tyrosine kinase Fes, and intersectin in CRMP function. Hyperphosphorylation of CRMP2 is an early event in the progression of Alzheimers disease.
Function:
Plays a role in neuronal development and polarity, as well as in axon growth and guidance, neuronal growth cone collapse and cell migration. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton (By similarity). Plays a role in neuron projection morphogenesis.
Subunit:
Homotetramer, and heterotetramer with CRMP1, DPYSL3, DPYSL4 or DPYSL5 (By similarity). Interacts through its C-terminus with the C-terminus of CYFIP1/SRA1. Interacts with HTR4 (By similarity). Interacts with CLN6.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Ubiquitous.
Post-translational modifications:
3F4, a monoclonal antibody which strongly stains neurofibrillary tangles in Alzheimer disease brains, specifically labels DPYSL2 when phosphorylated on Ser-518, Ser-522 and Thr-509.
Phosphorylation at Thr-514 by GSK3B abolishes tubulin-binding leading to destabilization of microtubule assembly in axons and neurodegeneration (By similarity). Phosphorylation by DYRK2 at Ser-522 is required for subsequent phosphorylation by GSK3B.
Similarity:
Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
SWISS:
Q16555
Gene ID:
1808
Database links:Entrez Gene: 1808 Human
Entrez Gene: 12934 Mouse
Entrez Gene: 25416 Rat
Omim: 602463 Human
SwissProt: Q16555 Human
SwissProt: O08553 Mouse
SwissProt: P47942 Rat
Unigene: 173381 Human
Unigene: 593187 Human
Unigene: 352648 Mouse
Unigene: 475100 Mouse
Unigene: 2889 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
CRMP2是一种脑特异性蛋白，对于轴突生长和轴突-树突发育起到很重要的作用，CRMP2通过与微管蛋白二聚体结合并促进微管的组装来调控轴突的生长和分支。
- 产品图片
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-CRMP2 (Thr514+Ser518)) Polyclonal Antibody, Unconjugated (bs-8284R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-CRMP2 (Thr514+Ser518)) Polyclonal Antibody, Unconjugated (bs-8284R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-CRMP2 (Thr514+Ser518)) Polyclonal Antibody, Unconjugated (bs-8284R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.