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货号： bs-0778R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0778R
- 英文名称
- CD90
- 中文名称
- CD90抗体
- 别 名
- CD90 / Thy1; CD7; CD90; CD90 antigen; CDw90; FLJ33325; MGC128895; T25; Theta antigen; Thy-1; Thy 1; Thy 1 cell surface antigen; Thy 1 membrane glycoprotein; Thy 1 membrane glycoprotein precursor; Thy 1.2; Thy-1 T-cell antigen; Thy1 antigen; Thy1 T cell antigen; Thy1.1; Thy1.2; Thymus cell antigen 1, theta; THY1_RAT; THY1_HUMAN.
- Specific References (12) | bs-0778R has been referenced in 12 publications.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 细胞生物 免疫学 神经生物学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Horse, Sheep,
- 产品应用
- WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 12kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from rat Thy-1:31-120/161 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Thy-1 or CD90 (Cluster of Differentiation 90) is a 25–37 kDa heavily N-glycosylated, glycophosphatidylinositol (GPI) anchored conserved cell surface protein with a single V-like immunoglobulin domain, originally discovered as a thymocyte antigen. Thy-1can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of Thy-1 lead to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to some of the initial biochemical description and characterization of a vertebrate GPI anchor and also the first demonstration of tissue specific differential glycosylation.
Function:
May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.
Subcellular Location:
Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Specificity:
Abundant in lymphoid tissues.
Post-translational modifications:
Glycosylation is tissue specific. Sialylation of N-glycans at Asn-93 in brain and at Asn-42, Asn-93 and Asn-117 in thymus.
Similarity:
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
SWISS:
P01830
Gene ID:
24832
Database links:Entrez Gene: 7070 Human
Entrez Gene: 21838 Mouse
Entrez Gene: 24832 Rat
Omim: 188230 Human
SwissProt: P04216 Human
SwissProt: P01831 Mouse
SwissProt: P01830 Rat
Unigene: 644697 Human
Unigene: 3951 Mouse
Unigene: 108198 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti-CD90 (bs-0778R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12 kD
Observed band size: 32 kD
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti-CD90 (bs-0778R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12 kD
Observed band size: 32 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Thy-1/CD90/ Thy1.1 Polyclonal Antibody, Unconjugated(bs-0778R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: U937(blue).
Primary Antibody: Rabbit Anti-CD90 antibody(bs-0778R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min).Primary antibody (bs-0778R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Blank control (blue line): U251 (blue).
Primary Antibody (green line): Rabbit Anti- CD90 antibody (bs-0778R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.