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货号： bs-4293R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-4293R
- 英文名称
- MACC1
- 中文名称
- 结肠癌转移相关蛋白1抗体
- 别 名
- 7A5; MACC1; MACC-1; MACC1_HUMAN; Metastasis associated in colon cancer 1; Metastasis-associated in colon cancer protein 1; Putative binding protein 7a5; SH3 domain-containing protein 7a5; SH3BP4L.
- Specific References (3) | bs-4293R has been referenced in 3 publications.[IF=1.48] Sun, Longfeng, et al. "Silence of MACC1 expression by RNA interference inhibits proliferation, invasion and metastasis, and promotes apoptosis in U251 human malignant glioma cells." Molecular Medicine Reports. WB ; Human.PubMed:26043756[IF=1.48] Li, Hongyu, et al. "Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer." Oncology letters9.5 (2015): 1989-1996. IHC-P ; Human.PubMed:26137000[IF=6.36] Montorsi, Lucia, et al. "Loss of zfp36 expression in colorectal cancer correlates to wnt/ß-catenin activity and enhances epithelial-to-mesenchymal transition through upregulation of zeb1, sox9 and macc1." Oncotarget (2016). WB ; Human.PubMed:27463018
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 肿瘤 免疫学 信号转导 生长因子和激素 转录调节因子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Cow,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 94kDa
- 细胞定位
- 细胞核 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human MACC1:761-852/852
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
MACC1 is a key regulator of the hepatocyte growth factor(HGF; MIM 142409)-HGF receptor (HGFR, or MET; MIM 164860) pathway, which is involved in cellular growth, epithelial-mesenchymal transition, angiogenesis, cell motility, invasiveness, and metastasis. Expression of MACC1 in colon cancer (MIM 114500) specimens is an independent prognostic indicator for metastasis formation and metastasis-free survival (Stein et al., 2009 [PubMed19098908]).[supplied by OMIM].
Subunit:
Interacts with FASLG.
Subcellular Location:
Cytoplasm. Nucleus. Note=Mainly found in the cytoplasm in non-metastasizing tumors.
Tissue Specificity:
Preferentially expressed in metastasizing tumors.
Similarity:
Contains 1 SH3 domain.
SWISS:
Q6ZN28
Gene ID:
346389
Database links:Entrez Gene: 346389 Human
Entrez Gene: 238455 Mouse
Entrez Gene: 314539 Rat
Omim: 612646 Human
SwissProt: Q6ZN28 Human
Unigene: 598388 Human
Unigene: 31376 Mouse
Unigene: 81938 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
liver(mouse) Lysate at 40 ug
Primary: Anti-MACC1 (bs-4293R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 94 kD
Observed band size: 94 kD
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MACC1 Polyclonal Antibody, Unconjugated(bs-4293R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MACC1) Polyclonal Antibody, Unconjugated (bs-4293R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.