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货号： bs-14552R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-14552R
- 英文名称
- eIF4GII
- 中文名称
- eIF4GII蛋白抗体
- 别 名
- eIF-4-gamma 3; eIF-4-gamma II; eIF-4G 3; eIF4G 3; EIF4G3; eIF4GII; Eukaryotic translation initiation factor 4 gamma 3; eukaryotic translation initiation factor 4 gamma, 3; eukaryotic translation initiation factor-4 gamma, 3; IF4G3_HUMAN.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物 转录调节因子 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human,
- 产品应用
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 177kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human eIF4GII:451-550/1585
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a 5’-cap motif and recruits the 43S ribosomal preinitiation complex to the transcript. Along with eIF4B, the eIF4F complex mediates the unwinding of mRNA secondary structure to facilitate ribosome association. eIF4E specifically interacts with the 5’ cap, eIF4A is a bidirectional RNA helicase, and eIF4GI and eIF4GII are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3 and the 40S ribosome. eIF4GII (also known as eIF4G3 and eIF4-g3) is a 1,585 amino acid protein that is 46% homologous and functionally similar to eIF4GI.
Function:
Probable component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5-terminal secondary structure and recruitment of mRNA to the ribosome. Thought to be a functional homolog of EIF4G1.
Subunit:
Interacts with EIF4A, EIF4E, eIF3 and PABPC1. Part of a complex with EIF4E. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4G1/EIF4G3 interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate eIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIFG3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA (By similarity).
Post-translational modifications:
Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.
Similarity:
Belongs to the eukaryotic initiation factor 4G family.
Contains 5 HEAT repeats.
Contains 1 MI domain.
Contains 1 MIF4G domain.Contains 1 W2 domain.
SWISS:
O43432
Gene ID:
8672
Database links:Entrez Gene: 8672Human
Entrez Gene: 230861Mouse
Entrez Gene: 298573Rat
Omim: 603929Human
SwissProt: O43432Human
SwissProt: Q80XI3Mouse
Unigene: 467084Human
Unigene: 732241Human
Unigene: 268903Mouse
Unigene: 407375Mouse
Unigene: 489798Mouse
Unigene: 220142Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.