Anti-phospho-TPH (Ser260)【科瑞奥博】

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货号： bs-5601R 基本售价： 1580.0 元规格： 100ul

别名: Tph1(phospho S260); TPH (phospho Ser260); p-TPH (Ser260); MGC119994; TPH 1; TPH; TPH1; TPRH; Tryptophan 5 hydroxylase 1; Tryptophan 5 monooxygenase 1; Tryptophan 5 monooxygenase; Tryptophan hydroxylase 1; Tryptophan 5-hydroxylase 1; TPH1_HUMAN.

产品应用: WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

免疫原: KLH conjugated Synthesised phosphopeptide derived from human TPH around the phosphorylation site of Ser260:HS(p-S)DP

保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品介绍: background:
This gene encodes a member of the aromatic amino acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene have been associated with an elevated risk for a variety of diseases and disorders, including schizophrenia, somatic anxiety, anger-related traits, bipolar disorder, suicidal behavior, addictions, and others.[provided by RefSeq, Apr 2009].

Subunit:
Homotetramer (By similarity).

Tissue Specificity:
Isoform 2 seems to be less widely expressed than isoform 1.

Similarity:
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Contains 1 ACT domain.

SWISS:
P17752

Gene ID:
7166

Database links:
Entrez Gene: 7166 Human
Entrez Gene: 21990 Mouse
Entrez Gene: 24848 Rat
Omim: 191060 Human
SwissProt: P17752 Human
SwissProt: P17532 Mouse
SwissProt: P09810 Rat
Unigene: 591999 Human
Unigene: 248684 Mouse
Unigene: 94788 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

色氨酸在脑中首先由色氨酸羟化酶(tryoptophan hydroxylase)催化生成5羟色氨酸(5hydroxytryptophan)，再经脱羧酶作用生成5羟色胺，色氨酸羟化酶（TPH）是5-HT生物合成的限速酶，因此，色氨酸羟化酶的缺少可导致5-羟色胺的含量下降。色氨酸羟化酶主要在肝脏内代谢。

产品图片: Sample:
Cerebrum(Rat) Lysate at 40 ug
Primary: Anti-phospho-TPH (Ser260) (bs-5601R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Sample: HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-TPH (Ser260) (bs-5601R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-phospho-TPH (Ser260) (bs-5601R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TPH (Ser260)) Polyclonal Antibody, Unconjugated (bs-5601R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.