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货号： bs-5496R 基本售价： 1580.0 元 规格： 100ul
产品信息
- 产品编号
- bs-5496R
- 英文名称
- phospho-MAP3K7IP1 (Thr431)
- 中文名称
- 磷酸化转化生长因子β活化激酶结合蛋白1抗体
- 别 名
- MAP3K7IP1(phospho S431); MAP3K7IP1(phospho S429) mo, rat; MAP3K7IP 1; MAP3K7IP1; Mitogen activated protein kinase kinase kinase 7 interacting protein 1; TAB 1; TAK1 binding protein 1; TGF beta activated kinase 1 binding protein 1; TGF-beta activated kinase 1/MAP3K7 binding protein 1; Transforming growth factor beta activated kinase binding protein 1; 2310012M03Rik; 3-Tab1; MGC57664; TAB1_HUMAN.
- 规格价格
- 100ul/1580元购买 大包装/询价
- 说 明 书
- 100ul
- 产品类型
- 磷酸化抗体
- 研究领域
- 肿瘤 细胞生物 免疫学 信号转导 转录调节因子 激酶和磷酸酶
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 56kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated Synthesised phosphopeptide derived from human MAP3K7IP1 around the phosphorylation site of Ser431:EA(p-T)PT
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
TAB1 was identified as a regulator of the MAP kinase kinase kinase TAK1/MAP3K7, which is known to mediate various intracellular signaling pathways, such as those induced by TGF beta and members of the Toll IL 1R (TIR) superfamily, thus acting as an intermediate in both proliferative and innate and adaptive immune responses. This protein, together with either TAB2 or TAB3, activates TAK1 kinase in response to upstream signals. It has been shown that the C terminal portion of TAB1 is sufficient for binding and activation of TAK1, while a portion of the N terminus acts as a dominant negative inhibitor of TGF beta, demonstrating how this protein can function as a mediator between TGF beta receptors and TAK1.
Function:
May be an important signaling intermediate between TGFB receptors and MAP3K7/TAK1. May play an important role in mammalian embryogenesis.
Subunit:
Interacts with XIAP and BIRC7. Interacts with TRAF6 and MAP3K7; during IL-1 signaling. Identified in the TRIKA2 complex composed of MAP3K7, TAB1 and TAB2.
Tissue Specificity:
Ubiquitous.
Post-translational modifications:
Monoubiquitinated. Deubiquitinated by Y.enterocolitica YopP.
Similarity:
Contains 1 PP2C-like domain.
SWISS:
Q15750
Gene ID:
10454
Database links:Entrez Gene: 10454 Human
Entrez Gene: 66513 Mouse
Entrez Gene: 315139 Rat
Omim: 602615 Human
SwissProt: Q15750 Human
SwissProt: Q8CF89 Mouse
Unigene: 507681 Human
Unigene: 288245 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
U937 Cell (Human) Lysate at 40 ug
Hela Cell (Human) Lysate at 40 ug
Siha Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-MAP3K7IP1 (Thr431)(bs-5496R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56kD
Observed band size: 65kD
Sample: 293T Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-MAP3K7IP1 (Thr431)(bs-5496R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56kD
Observed band size: 60kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-MAP3K7IP1 (Thr431)) Polyclonal Antibody, Unconjugated (bs-5496R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.