Anti-phospho-IGF1R (Tyr980)【科瑞奥博】

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Anti-phospho-IGF1R (Tyr980)

货号： bs-5447R 基本售价： 1580.0 元规格： 100ul

产品信息

产品编号: bs-5447R

英文名称: phospho-IGF1R (Tyr980)

中文名称: 磷酸化胰岛素样生长因子1受体抗体

别名: IGF1 Receptor (phospho Y980); p-IGF1 Receptor (phospho Y980); IGF1R (phospho-Tyr980); IGF1R (phospho-Y980); p-IGF1R (Tyr980); p-IGF1R (Y980); IGF1R (phospho Tyr980); CD221; CD221; CD221 antigen; IGF 1 receptor; IGF 1R; IGF 1R; IGF I receptor; IGF-I receptor; IGF1R; IGF1R; IGF1R_HUMAN; IGFIR; IGFIRC; IGFIRC; IGFR; Insulin like growth factor 1 receptor; Insulin like growth factor 1 receptor; Insulin like growth factor 1 receptor precursor; Insulin like growth factor 1 receptor precursor; Insulin-like growth factor 1 receptor beta chain; Insulin-like growth factor I receptor; JTK13; JTK13; MGC142170; MGC142172; MGC18216; IGF-IR.

: Specific References  (2)     |     bs-5447R has been referenced in 2 publications.
[IF=6.20] Tang, Hexiao, et al. "Estrogen and insulin?\like growth factor 1 synergistically promote the development of lung adenocarcinoma in mice."International Journal of Cancer (2013)..  WB ;  Mouse.
PubMed:23649836
[IF=4.77] Lee, Yongik, et al. "Inhibition of IGF1R signaling abrogates resistance to afatinib (BIBW2992) in EGFR T790M mutant lung cancer cells." Molecular Carcinogenesis (2015).  WB ;  Human.
PubMed:26052929

规格价格: 100ul/1580元购买大包装/询价

说明书: 100ul

产品类型: 磷酸化抗体

研究领域: 免疫学生长因子和激素转录调节因子激酶和磷酸酶细胞膜受体糖尿病

抗体来源: Rabbit

克隆类型: Polyclonal

交叉反应: Human, Mouse, Rat,

产品应用: WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量: 150kDa

细胞定位: 细胞膜

性状: Lyophilized or Liquid

浓度: 1mg/ml

免疫原: KLH conjugated Synthesised phosphopeptide derived from human IGF1R around the phosphorylation site of Tyr980:PE(p-Y)FS

亚型: IgG

纯化方法: affinity purified by Protein A

储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed: PubMed

产品介绍

background:
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq, Jul 2008].

Function:
This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.

Subunit:
Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Forms a hybrid receptor with INSR, the hybrid is a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R. Interacts with ARRB1 and ARRB2.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibrobasts, spleen and placenta (at protein level). Expressed in a variety of tissues.

Post-translational modifications:
The cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin-like growth factor I (IGF I).
Phosphorylation of Tyr-980 is required for IRS1- and SHC1-binding.

DISEASE:
Defects in IGF1R may be a cause in some cases of resistance to insulin-like growth factor 1 (IGF1 resistance) [MIM:270450]. IGF1 resistance is a gowth deficiency disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1.

Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Insulin receptor subfamily.
Contains 3 fibronectin type-III domains.
Contains 1 protein kinase domain.

SWISS:
P08069

Gene ID:
3480

Database links:

Entrez Gene: 3480 Human

Entrez Gene: 16001 Mouse

Entrez Gene: 25718 Rat

Omim: 147370 Human

SwissProt: P08069 Human

SwissProt: Q60751 Mouse

SwissProt: P24062 Rat

Unigene: 643120 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片: Tissue/cell: human kidney carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-IGF1R(Tyr980) Polyclonal Antibody, Unconjugated(bs-5447R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
The blue histogram is unstained cells(Hepg2 cells).
The green histogram is cells stained with Rabbit Anti-phospho-IGF1R (Tyr980) antibody (bs-5447R) plus secondary antibody（bs-0295G-FITC).Concentration 1:20
Blank control(blue): Mouse Kidney (fixed with 2% paraformaldehyde for 10 min at 37℃).
Primary Antibody:Rabbit Anti-phospho-IGF1R (Tyr980) antibody (bs-5447R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.