Anti-Legumain【科瑞奥博】

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Anti-Legumain

货号： bs-3907R 基本售价： 1380.0 元规格： 100ul

规格：100ul: 价格：1380.00元

规格：200ul: 价格：2200.00元

产品信息

产品编号: bs-3907R

英文名称: Legumain

中文名称: 半胱氨酸蛋白酶抗体

别名: AEP; Asparaginyl endopeptidase; EC 3.4.22.34; LGMN; LGMN1; Protease, cysteine 1; protease, cysteine, 1 (legumain); PRSC1; LGMN_HUMAN.

规格价格: 100ul/1380元购买 200ul/2200元购买大包装/询价

说明书: 100ul 200ul

研究领域: 肿瘤细胞生物免疫学细胞凋亡激酶和磷酸酶

抗体来源: Rabbit

克隆类型: Polyclonal

交叉反应: Human, Mouse, Rat, Dog, Cow, Horse,

产品应用: WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量: 46kDa

细胞定位: 细胞浆

性状: Lyophilized or Liquid

浓度: 1mg/ml

免疫原: KLH conjugated synthetic peptide derived from human Legumain:201-300/433

亚型: IgG

纯化方法: affinity purified by Protein A

储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed: PubMed

产品介绍

background:
This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008].

Function:
Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

Subcellular Location:
Lysosome (By similarity).

Tissue Specificity:
Ubiquitous. Particularly abundant in kidney, heart and placenta.

Post-translational modifications:
Glycosylated.

Similarity:
Belongs to the peptidase C13 family.

SWISS:
Q99538

Gene ID:
5641

Database links:

Entrez Gene: 5641 Human

Entrez Gene: 19141 Mouse

Entrez Gene: 63865 Rat

Omim: 602620 Human

SwissProt: Q99538 Human

SwissProt: O89017 Mouse

SwissProt: Q9R0J8 Rat

Unigene: 726036 Human

Unigene: 17185 Mouse

Unigene: 206021 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片: Sample: Placenta (Mouse) Lysate at 40 ug
Primary: Anti-Legumain (bs-3907R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 55 kD
Sample: Kidney (Mouse) Lysate at 40 ug
Primary: Anti- Legumain (bs-3907R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 55 kD
Blank control: HL60. Primary Antibody (green line): Rabbit Anti-Legumain antibody (bs-3907R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG .Secondary Antibody : Goat anti-rabbit IgG-PEDilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Overlay histogram showing Mouse spleen cells stained with bs-3907-PE (red line). The cells were fixed with 1% paraformaldehyde (10 min).The cells were then incubated with the antibody (bs-3907R-PE, 2ug/1x10⁶cells) for 30 min at 22-25℃. Isotype control antibody (black line) was rabbit IgG (2ug/1x10⁶cells) used under the same conditions. Acquisition of events were used for analysis.