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货号： bs-3889R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-3889R
- 英文名称
- NOX2
- 中文名称
- NADPH氧化酶2抗体
- 别 名
- AMCBX2; CGD; CGD91-phox; CY24B_HUMAN; CYBB; Cytochrome b 245, beta polypeptide; Cytochrome b(558) beta chain; Cytochrome b(558) subunit beta; Cytochrome b-245 heavy chain; Cytochrome b558 subunit beta; GP91 PHOX; gp91-1; gp91-phox; GP91PHOX; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; p22 phagocyte B-cytochrome; P91 PHOX; p91-PHOX; Superoxide-generating NADPH oxidase heavy chain subunit.
- Specific References (6) | bs-3889R has been referenced in 6 publications.[IF=3.21] Dalaklioglu, Selvinaz, et al. "Metastatic breast carcinoma induces vascular endothelial dysfunction in Balb-c mice: Role of the tumor necrosis factor-?? and NADPH oxidase."Vascular Pharmacology (2013).& Mouse.PubMed:23933568[IF=7.19] Wang, Yabin, et al. "Activation of Liver X receptor improves viability of adipose-derived mesenchymal stem cells (AD-MSCs) to attenuate myocardial ischemia injury through TLR4/NF-κB and Keap-1/Nrf2 signaling pathways." Antioxidants & Redox Signaling ja (2014). WB ; Mouse.PubMed:24915051[IF=1.25] Kashiwabara, Yoshiaki, et al. "Immunohistochemical localization of Nox in mouse circumvallate papillae." Tissue and Cell (2015). IHC-P ; Mouse.PubMed:26603975[IF=9.83] Wakatsuki, Shuji, et al. "Oxidative stress–dependent phosphorylation activates ZNRF1 to induce neuronal/axonal degeneration." The Journal of Cell Biology (2015): jcb-201506102. IHC-P ; Mouse.PubMed:26572622[IF=0.00] Hu, Xiaoqin, et al. "Effects of Resveratrol on Oxidative Stress Injury Induced by Rapid-Pacing in Isolated Rabbit Hearts." Journal of Developing Drugs 3.128 (2014): 2. WB ; Rabbit.PubMed:0[IF=25.56] Lagadinou, Eleni D., et al. "BCL-2 inhibition targets oxidative phosphorylation and selectively eradicates quiescent human leukemia stem cells." Cell stem cell 12.3 (2013): 329-341. FCM ; Human.PubMed:23333149
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 免疫学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 65kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human NOX2:501-570/570
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
NOX2/gp91phox is a critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. It also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. Defects in CYBB are a cause of X-linked chronic granulomatous disease (X-CGD). X-CGD is characterized by the failure of activated phagocytes to generate superoxide. Patients suffer from life-threatening bacterial/fungal infections.
Function:
Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
Subcellular Location:
Membrane.
Post-translational modifications:
Glycosylated.
DISEASE:
Defects in CYBB are a cause of chronic granulomatous disease X-linked (XCGD) [MIM:306400]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
Similarity:
Contains 1 FAD-binding FR-type domain.
Contains 1 ferric oxidoreductase domain.
SWISS:
P04839
Gene ID:
1536
Database links:Entrez Gene: 281112 Cow
Entrez Gene: 1536 Human
Entrez Gene: 13058 Mouse
Entrez Gene: 100008801 Rabbit
Entrez Gene: 66021 Rat
Omim: 300481 Human
SwissProt: O46522 Cow
SwissProt: P04839 Human
SwissProt: Q61093 Mouse
Unigene: 292356 Human
Unigene: 200362 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
NOX2也是一个很重要的产生氧自由基的酶。主要存在于甲状腺,前列腺，唾液腺和直肠粘膜中。
- 产品图片
Sample: Spleen (Mouse) Lysate at 40 ug
Primary: Anti-NOX2 (bs-3889R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
Sample: Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-NOX2 (bs-3889R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NOX2 Polyclonal Antibody, Unconjugated(bs-3889R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei