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货号： bs-3673R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-3673R
- 英文名称
- DIO2
- 中文名称
- 脱碘酶2抗体
- 别 名
- 5DII; D2; Deiodinase, iodothyronine, type II; DIOII; ITDI2; SelY; Thyroxine deiodinase, type II; TXDI2; Type 2 DI; Type II 5 deiodinase; Type II iodothyronine deiodinase.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 免疫学 生长因子和激素 内分泌病
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 30kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human DIO2:101-200/273
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
DIO2 belongs to the iodothyronine deiodinase family and is responsible for the deiodination of T4 (3,5,3,5-tetraiodothyronine) into T3 (3,5,3-triiodothyronine). It is essential for providing the brain with appropriate levels of T3 during the critical period of development. DIO2 is expressed in heart, skeletal muscle, placenta, fetal brain and several regions of the adult brain. There are two named isoforms.
Function:
Responsible for the deiodination of T4 (3,5,3,5-tetraiodothyronine) into T3 (3,5,3-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.
Subunit:
Interacts with USP20 and USP33. Interacts with MARCH6.
Subcellular Location:
Membrane; Single-pass membrane protein (Potential).
Tissue Specificity:
Heart, skeletal muscle, placenta, fetal brain and several regions of the adult brain.
Post-translational modifications:
Ubiquitinated by MARCH6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33.
Similarity:
Belongs to the iodothyronine deiodinase family.
SWISS:
Q92813
Gene ID:
1734
Database links:Entrez Gene: 494548 Cow
Entrez Gene: 1734 Human
Entrez Gene: 13371 Mouse
Entrez Gene: 414379 Pig
Entrez Gene: 65162 Rat
Omim: 601413 Human
SwissProt: Q5I3B2 Cow
SwissProt: Q92813 Human
SwissProt: Q9Z1Y9 Mouse
SwissProt: Q6QN12 Pig
SwissProt: P70551 Rat
Unigene: 202354 Human
Unigene: 21389 Mouse
Unigene: 408815 Mouse
Unigene: 88380 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
脱碘酶2泛称Ⅱ型碘甲腺氨酸脱碘酶脱碘，是调节甲状腺激素生物活性的特别方式。脱碘酶可加速T4转化为T3，引起甲状腺激素及生长激素分泌减少，影响一些机体生物学的改变。 DIO2主要表达在中枢神经系统、垂体前叶腺体、棕色脂肪组织和胎盘内。其他组织中也有一定量的分布：心肌和骨胳肌、肝、肾、中枢神经系统、 脑，皮肤，胎盘等。
- 产品图片
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-DIO2 (bs-3673R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DIO2) Polyclonal Antibody, Unconjugated (bs-3673R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DIO2) Polyclonal Antibody, Unconjugated (bs-3673R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.