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货号： bs-3669R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-3669R
- 英文名称
- BRN3A
- 中文名称
- 转录因子Brn3蛋白抗体
- 别 名
- Brain specific homeobox/POU domain protein 3A; Brn 3.0; BRN 3A; Brn3; Homeobox/POU domain protein RDC 1; Homeobox/POU domain protein RDC1; Oct T1; OctT1; POU Domain Class 4 Transcription Factor 1; POU4F1; RDC 1; RDC1. Brn3α;
- Specific References (2) | bs-3669R has been referenced in 2 publications.[IF=16.09] Xue, Yuanchao, et al. "Sequential regulatory loops as key gatekeepers for neuronal reprogramming in human cells." Nature Neuroscience (2016). IF(ICC) ; Human.PubMed:27110916[IF=3.84] Bell, Katharina, et al. "Neuroprotective effects of antibodies on retinal ganglion cells in an adolescent retina organ culture." Journal of Neurochemistry (2016). IHC-P ; Pig.PubMed:27507598
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 免疫学 神经生物学 转录调节因子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 43kDa
- 细胞定位
- 细胞核
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human BRN3A:325-419/419
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes a member of the POU-IV class of neural transcription factors. This protein is expressed in a subset of retinal ganglion cells and may be involved in the developing sensory nervous system. This protein may also promote the growth of cervical tumors. A translocation of this gene is associated with some adult acute myeloid leukemias. [provided by RefSeq, Mar 2012].
Function:
Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons.
Subcellular Location:
Nucleus.
Tissue Specificity:
Brain. Seems to be specific to the retina. Present in the developing brain, spinal cord and eye.
DISEASE:
Expression peaks early in embryogenesis (day 13.5) and is undetectable 14 days after birth.
Similarity:
Belongs to the POU transcription factor family. Class-4 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
SWISS:
Q01851
Gene ID:
5457
Database links:Entrez Gene: 5457Human
Entrez Gene: 18996Mouse
Entrez Gene: 114503Rat
Omim: 601632Human
SwissProt: Q01851Human
SwissProt: P17208Mouse
SwissProt: P20266Rat
Unigene: 654522Human
Unigene: 246550Mouse
Unigene: 232568Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
转录因子Brn3可能直接参与对轴突和树突生长能力的调控。
- 产品图片
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Spinal cord (Mouse) Lysate at 40 ug
Primary: Anti-BRN3A (bs-3669R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 33 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BRN3A Polyclonal Antibody, Unconjugated(bs-0071R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with BRN3A Antibody(bs-3669R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).