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货号： bs-3608R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-3608R
- 英文名称
- CYP11A1
- 中文名称
- 细胞色素P450 11A1抗体
- 别 名
- Cholesterol 20 22 desmolase； Cholesterol desmolase； Cholesterol monooxygenase (side chain cleaving)； Cholesterol side chain cleavage enzyme； Cholesterol side chain cleavage enzyme mitochondrial； Cholesterol side-chain cleavage enzyme； CP11A_HUMAN； CYP11A； CYP11A1； CYPXIA1； Cytochrome P450 11A1； Cytochrome P450 11A1 mitochondrial； Cytochrome P450 family 11 subfamily A polypeptide 1； Cytochrome P450 subfamily XIA； Cytochrome P450(scc)； Cytochrome P450C11A1； mitochondrial； P450SCC； Steroid 20 22 lyase.
- Specific References (3) | bs-3608R has been referenced in 3 publications.[IF=2.14] Hu, Yanhui, et al. "Low-dose monobutyl phthalate stimulates steroidogenesis through steroidogenic acute regulatory protein regulated by SF-1, GATA-4 and C/EBP-beta in mouse Leydig tumor cells." Reproductive Biology and Endocrinology 11.1 (2013): 72. WB ; Mouse.PubMed:23889939[IF=2.60] Mahata, Bidesh, et al. "Single-Cell RNA Sequencing Reveals T Helper Cells Synthesizing Steroids De Novo to Contribute to Immune Homeostasis." Cell Reports (2014). Mouse.PubMed:24813893[IF=2.08] Medwid, Samantha, Haiyan Guan, and Kaiping Yang. "Prenatal exposure to bisphenol A disrupts adrenal steroidogenesis in adult mouse offspring."Environmental Toxicology and Pharmacology (2016). WB ; Mouse.PubMed:27017381
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 免疫学 转录调节因子 线粒体
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 53/57kDa
- 细胞定位
- 细胞浆 细胞膜 线粒体
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human CYP11A1/P450SCC:321-420/521
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008]
Function:
Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.
Subcellular Location:
Mitochondrion membrane.
DISEASE:
Defects in CYP11A1 are the cause of adrenal insufficiency congenital with 46,XY sex reversal (AICSR) [MIM:613743]. A rare disorder that can present as acute adrenal insufficiency in infancy or childhood. ACTH and plasma renin activity are elevated and adrenal steroids are inappropriately low or absent; the 46,XY patients have female external genitalia, sometimes with clitoromegaly. The phenotypic spectrum ranges from prematurity, complete underandrogenization, and severe early-onset adrenal failure to term birth with clitoromegaly and later-onset adrenal failure. Patients with congenital adrenal insufficiency do not manifest the massive adrenal enlargement typical of congenital lipoid adrenal hyperplasia.
Similarity:
Belongs to the cytochrome P450 family.
SWISS:
P05108
Gene ID:
1583
Database links:Entrez Gene: 1583 Human
Omim: 118485 Human
SwissProt: P05108 Human
Unigene: 303980 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample: Placenta (Mouse) Lysate at 30 ug
Primary: Anti- CYP11A1 (Ser576) (bs-3608R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 53/57 kD
Observed band size: 57 kD
Sample:
Lane1: Brain(Rat) Lysate at 30 ug
Lane2: Kidney(Rat)Lysate at 30 ug
Primary: Anti-CYP11A1/P450SCC (bs-3608R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 53kD
Observed band size : 53kD
Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CYP11A1) Polyclonal Antibody, Unconjugated (bs-3608R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.