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货号： bs-0384R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0384R
- 英文名称
- GLUT4
- 中文名称
- 葡萄糖转运蛋白4抗体
- 别 名
- insulin-responsive; Glucose transporter GLUT 4; Glucose Transporter GLUT4; Glucose transporter type 4; Glucose transporter type 4 insulin responsive; GLUT 4; GLUT-4; GLUT4; GTR4_HUMAN; kug; SLC 2A4; SLC2A4; solute carrier family 2 (facilitated glucose transporter) member 4; Solute carrier family 2, facilitated glucose transporter member 4.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 心血管 免疫学 信号转导 干细胞 糖尿病
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 54kDa
- 细胞定位
- 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human GLUT4:401-509/509 <Cytoplasmic>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
GLUT4 is the facilitated glucose transporter expressed exclusively in adipocytes and muscle cells, and is also known as the "insulin-responsive" glucose transporter. GLUT4 translocates from an ill-defined intracellular compartment to the plasma membrane in response to insulin. The total cellular content of GLUT4 is significantly decreased in adipose cells from many patients with Type II diabetes mellitus, and animals with some types of experimental diabetes.
Function:
Insulin-regulated facilitative glucose transporter.
Subcellular Location:
Endomembrane system. Cytoplasm > perinuclear region. Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.
Tissue Specificity:
Skeletal and cardiac muscles; brown and white fat.
Post-translational modifications:
Sumoylated.
DISEASE:
Defects in SLC2A4 may be a cause of noninsulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. Defects in SLC2A4 may be a cause of certain post-receptor defects in NIDDM. The variant in position Ile-383 is found in a small number of NIDDM patients, but seems not to be found in nondiabetic subjects.
Similarity:
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
SWISS:
P14672
Gene ID:
6517
Database links:Entrez Gene: 282359 Cow
Entrez Gene: 6517 Human
Entrez Gene: 20528 Mouse
Entrez Gene: 25139 Rat
Omim: 138190 Human
SwissProt: Q27994 Cow
SwissProt: Q29RP5 Cow
SwissProt: P14672 Human
SwissProt: P14142 Mouse
SwissProt: P19357 Rat
Unigene: 380691 Human
Unigene: 10661 Mouse
Unigene: 1314 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
葡萄糖转运蛋白-4是一种十分重要的葡萄糖转运体,与胰岛素抵抗和2型糖尿病密切相关.葡萄糖转运蛋白4在细胞内部和细胞膜之间循环流动.实现对葡萄糖的转运需要葡萄糖转运蛋白4自身的转位和活化.葡萄糖转运蛋白4与肥胖、肿瘤相关联.
GLUT-2和GLUT-4蛋白这两个葡萄糖运载体的研究对于糖尿病的胰岛素释放障碍和胰岛素抵抗有重要意义.
- 产品图片
Sample:
Heart(Rat) Cell Lysate at 40 ug
Primary: Anti-GLUT4 (bs-0384R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Sample:
muscle (mouse) Lysate at 40 ug
heart (mouse) Lysate at 40 ug
Primary: Anti- beta tubulin(bs-0384R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 54 kD
Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti- GLUT4 (bs-0384R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 54 kD
Observed band size: 54 kD
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GLUT4 Polyclonal Antibody, Unconjugated(bs-0384R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GLUT4 Polyclonal Antibody, Unconjugated(bs-0384R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line): K562 (blue).
Primary Antibody (green line): Rabbit Anti-GLUT4 antibody (bs-0384R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 70% ethanol (overnight at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.