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货号： bs-20745R-FITC 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-20745R-FITC
- 英文名称
- Anti-Nurr1/FITC
- 中文名称
- FITC标记的核受体相关因子-1抗体
- 别 名
- Nur-ralated factor 1; Nuclear receptor-related factor 1; Orphan nuclear receptor NR4A2; Orphan nuclear receptor NURR1; Regenerating liver nuclear receptor 1; RNR-1; SL-322; Nuclear orphan receptor HZF-3; T-cell nuclear receptor NOT;HZF 3; HZF3; Immediate-early response protein NOT; Intermediate early receptor protein; NGFI B/nur77 beta type transcription factor homolog; NOT; NR4A2; NR4A2_HUMAN; Nuclear receptor subfamily 4 group A member 2; Nur related protein 1 homolog; Nurr 1;RNR 1; RNR1; TINUR; Transcriptionally inducible nuclear receptor; Transcriptionally inducible nuclear receptor related; Transcriptionally inducible nuclear receptor related 1; Transcriptionally-inducible nuclear receptor.
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 染色质和核信号 神经生物学 新陈代谢 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
- 产品应用
- ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 67kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 2mg/1ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Nurr1
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
Nuclear receptor and transcription factor; plays a role in development and maintenance of neurons synthesizing the neurotransmitter dopamine This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. The encoded protein may act as a transcription factor. Mutations in this gene have been associated with disorders related to dopaminergic dysfunction, including Parkinson disease, schizophernia, and manic depression. Misregulation of this gene may be associated with rheumatoid arthritis. Four transcript variants encoding four distinct isoforms have been identified for this gene. Additional alternate splice variants may exist, but their full length nature has not been determined.
Function:
Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. It is crucial for expression of a set of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons.
Subunit:
Interacts with SFPQ, NCOR2, SIN3A and HADC1. The interaction with NCOR2 increases in the absence of PITX3.
Subcellular Location:
Nucleus.
Tissue Specificity:
Expressed in a number of cell lines of T-cell, B-cell and fibroblast origin. Strong expression in brain tissue.
Similarity:
Belongs to the nuclear hormone receptor family. NR4 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Database links:Entrez Gene: 4929 Human
Entrez Gene: 18227 Mouse
Entrez Gene: 54278 Rat
Omim: 601828 Human
SwissProt: P43354 Human
SwissProt: Q06219 Mouse
SwissProt: Q07917 Rat
Unigene: 563344 Human
Unigene: 3507 Mouse
Unigene: 88129 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
该抗体和类固醇/甲状腺素受体密切相关的早期应急反应基因，也受各种生长因子的诱导。中脑多巴胺神经细胞和脑干细胞表达。
- 产品图片
Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-Nurr1 antibody (bs-20745-FITC)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.