产品中心
当前位置：首页>产品中心Anti-Phospho-Jak1 (Tyr1034 + Tyr1035)
货号： bs-3238R 基本售价： 1580.0 元 规格： 100ul
产品信息
- 产品编号
- bs-3238R
- 英文名称
- Phospho-Jak1 (Tyr1034 + Tyr1035)
- 中文名称
- 磷酸化蛋白质酪氨酸激酶JAK-1抗体
- 别 名
- JAK1 (phospho Y1034 + Y1035); p-JAK1 (phospho Y1034 + Y1035); JAK 1; JAK 1A; JAK 1B; JAK-1; JAK1; JAK1_HUMAN; JAK1A; JAK1B; Janus kinase 1 (a protein tyrosine kinase); Janus kinase 1; JTK3; Tyrosine protein kinase JAK 1; Tyrosine protein kinase JAK1; Tyrosine-protein kinase JAK1.
- 规格价格
- 100ul/1580元购买 大包装/询价
- 说 明 书
- 100ul
- 产品类型
- 磷酸化抗体
- 研究领域
- 肿瘤 细胞生物 免疫学 染色质和核信号 信号转导 细胞凋亡 激酶和磷酸酶 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Pig, Cow,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=2μg /Test （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 133kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated Synthesised phosphopeptide derived from human Jak1 around the phosphorylation site of Tyr1034 + Tyr1035:KE(p-Y)(p-Y)TV
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Janus kinase 1 (JAK1) is a member of a new class of non-receptor protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. It is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon recpeptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs.
Function:
Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
Subunit:
Interacts with FER. Interacts with IL31RA, IFNAR2, JAKMIP1 and SHB.
Subcellular Location:
Endomembrane system; Peripheral membrane protein. Note=Wholly intracellular, possibly membrane associated.
Tissue Specificity:
Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
SWISS:
P23458
Gene ID:
3716
Database links:Entrez Gene: 3716 Human
Entrez Gene: 442952 Dog
Entrez Gene: 16451 Mouse
Entrez Gene: 84598 Rat
Omim: 147795 Human
SwissProt: P23458 Human
SwissProt: P52332 Mouse
Unigene: 207538 Human
Unigene: 289657 Mouse
Unigene: 90191 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:A549 (Human)cell Lysate at 40 ug
Primary: Anti-p-Jak1(Tyr1034 + Tyr1035)(bs-3238R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 133kD
Observed band size: 133kD
From 《Cancer Medicine》(2016.6): PublitionDirect effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes , IF:2.5
Author: Noriyoshi Iriyama, Yoshihiro Hatta & Masami Takei
Division of Hematology and Rheumatology, Department of Medicine, Nihon University School of Medicine, Tokyo, Japan
Blank control: HUVEC.
Primary Antibody (green line): Rabbit Anti-Phospho-Jak1 antibody (bs-3238R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.