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货号： bs-0325R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0325R
- 英文名称
- GAD65
- 中文名称
- 谷氨酸脱羧酶-65抗体
- 别 名
- GAD65; 65 kDa glutamic acid decarboxylase; DCE 2; DCE2; GAD 2; GAD 65; GAD-2; GAD-65; GAD2; Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa); Glutamate Decarboxylase 2; Glutamate Decarboxylase 65; Glutamate decarboxylase 65 kDa isoform; Glutamic Acid Decarboxylase 2; Glutamic Acid Decarboxylase 65.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 免疫学 糖尿病
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Chicken, Pig,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 65kDa
- 细胞定位
- 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human GAD65:501-585/585
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]
Function:
Catalyzes the production of GABA.
Subunit:
Homodimer.
Subcellular Location:
Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters.
Post-translational modifications:
Phosphorylated; which does not affect kinetic parameters or subcellular location.
Palmitoylated; which is required for presynaptic clustering.
Similarity:
Belongs to the group II decarboxylase family.
SWISS:
Q05329
Gene ID:
2572
Database links:Entrez Gene: 2572 Human
Entrez Gene: 14417 Mouse
Entrez Gene: 24380 Rat
Omim: 138275 Human
SwissProt: Q05329 Human
SwissProt: Q5VZ29 Human
SwissProt: Q5VZ30 Human
SwissProt: P48320 Mouse
SwissProt: Q548L4 Mouse
SwissProt: Q05683 Rat
Unigene: 231829 Human
Unigene: 4784 Mouse
Unigene: 29951 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
谷氨酸脱羧酶-65(GAD65)是用于I II型糖尿病研究的很重要的蛋白。
- 产品图片
Sample: Hela Cell Lysate at 40 ug
Primary: Anti- GAD65 (bs-0325R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
Sample: Lung(Rat) lysate at 30 ug;
Primary: Anti-GAD65 (bs-0325R) at 1:300 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size : 65kD
Observed band size : 65kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-GAD65 (bs-0325R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
Sample:
brain (Mouse) Lysate at 40 ug
Primary: Anti-GAD65 (bs-0325R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 65 kD
Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GAD65 Polyclonal Antibody, Unconjugated(bs-0325R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAD65) Polyclonal Antibody, Unconjugated (bs-0325R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.