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货号： bs-2754R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-2754R
- 英文名称
- Afadin/I Afadin
- 中文名称
- 丝状肌动蛋白结合蛋白抗体
- 别 名
- AF 6; AF6; AFAD_HUMAN; Afadin; ALL1-fused gene from chromosome 6 protein; FLJ34371; MLLT 4; MLLT4; Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog Drosophila) translocated to 4; Myeloid/lymphoid or mixed lineage leukemia translocated to 4; Protein AF 6; Protein Af-6; Protein AF6; RP3 431P23.3; 5033403D15Rik; Gm314; I-afadin; Mllt4; S-afadin.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 神经生物学 信号转导 细胞粘附分子 细胞骨架
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 136kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human AF6:341-440/1816
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Afadin is a cell-cell adherens junction F-actin binding multidomain protein. Two splice variants of afadin have been described: l-afadin, which is ubiquitously expressed, and the smaller s-afadin, which is abundantly expressed in neural tissue. l-Afadin contains one PDZ domain near its middle followed by three proline-rich and one F-actin-binding region at the carboxyl-terminal. l/s-Afadin is localized to cell-cell junctions and binds several cytoplasmic proteins such as the small GTPase Ras, the tight junction protein ZO-1, and the vinculin binding protein ponsin/SH3P12.
Function:
Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.
Subunit:
Homodimer. Interacts with F-actin, nectin and PVRL3/nectin-3. Essential for the association of nectin and E-cadherin. Isoform 1/s-afadin does not interact with F-actin. Interacts with ZO-1 and occludin, but probably in an indirect manner. Interacts with RIT1 and RIT2 (By similarity). Interacts with NRXN1 and BCR.
Subcellular Location:
Cell junction, adherens junction. Note=Not found at cell-matrix AJs.
DISEASE:
Note=A chromosomal aberration involving MLLT4 is associated with acute leukemias. Translocation t(6;11)(q27;q23) with MLL/HRX. The result is a rogue activator protein.
Similarity:
Contains 1 dilute domain.
Contains 1 FHA domain.
Contains 1 PDZ (DHR) domain.
Contains 2 Ras-associating domains
SWISS:
P55196
Gene ID:
4301
Database links:Entrez Gene: 4301 Human
Entrez Gene: 17356 Mouse
Entrez Gene: 26955 Rat
Omim: 159559 Human
SwissProt: P55196 Human
SwissProt: Q9QZQ1 Mouse
SwissProt: O35889 Rat
Unigene: 614974 Human
Unigene: 644024 Human
Unigene: 708726 Human
Unigene: 728849 Human
Unigene: 59167 Mouse
Unigene: 58 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Skin (Rat) Lysate at 40 ug
Primary: Anti-Afadin (bs-2754R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 136 kD
Observed band size: 135/138 kD
Sample:
Hippocampus (Mouse) Lysate at 40 ug
Primary: Anti-Afadin (bs-2754R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 136 kD
Observed band size: 135/138 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Afadin) Polyclonal Antibody, Unconjugated (bs-2754R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.