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货号： bs-2217R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-2217R
- 英文名称
- Integrin alpha E
- 中文名称
- 整合素alpha E2 抗体
- 别 名
- Itgae; A530055J10; alpha-E1; alpha-M290; aM290; CD103; MGC183601; antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide.ITAE_HUMAN; Integrin alpha-E; HML-1 antigen; Integrin alpha-IEL; Mucosal lymphocyte 1 antigen; Integrin alpha-E heavy chain; integrin alpha-E precursor.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物 免疫学 信号转导 干细胞 细胞粘附分子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-F=1:400-800 Flow-Cyt=1ug/test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 110/128kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Integrin alpha-E heavy chain:1101-1179/1179 <Cytoplasmic>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
CD103/Integrin alpha E is a new member of the integrin series of adhesion molecules containing the beta7 subunit. This antigen defines a developmentally important subset of T cells, namely mucosal T cells including all IEL (intraepithelial lymphocytes) and ~20% of lamina propria T cells. Expression of CD103 is more restricted outside these mucosal organs, appearing at lower levels on T cell subsets of the lymph node, dendritic epidermis and periphery. In non-epithelial CD103+ T cells there is a bias toward expression on CD8+ cells. Integrin alpha E is a heterodimer comprising a 150kDa alpha chain and a 120kDa beta chain. Integrin alpha E is expressed by approximately 1% of peripheral blood lymphocytes, activated T lymphocytes and by hairy cell leukaemia cells. The antigen is also expressed by intraepithelial lymphocytes.
Function:
Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers.
Subunit:
Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chains linked by a disulfide bond. Alpha-E associates with beta-7.
Subcellular Location:
Membrane; Single-pass type I membrane protein.Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells.
Tissue Specificity:
Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells.
Similarity:
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
SWISS:
P38570
Gene ID:
3682
Database links:Entrez Gene: 3682 Human
Omim: 604682 Human
SwissProt: P38570 Human
Unigene: 513867 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-Integrin alpha E2 (bs-2217R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110/128 kD
Observed band size: 128 kD
Sample:
U937(Human) Cell Lysate at 30 ug
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-Integrin alpha E2 (bs-2217R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110/128 kD
Observed band size: 110 kD
Molt-4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD103 Antibody(bs-2217R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).