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货号： bs-2684R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-2684R
- 英文名称
- GPNMB
- 中文名称
- GPNMB抗体
- 别 名
- NMB; Glycoprotein nmb like protein; Gpnmb; DC-HIL; Dchil; ipd; Glycoprotein (transmembrane) nmb; Glycoprotein nmb; HGFIN; Transmembrane glycoprotein; Transmembrane glycoprotein HGFIN; Transmembrane glycoprotein NMB; Osteoactivin; GPNMB_HUMAN.
- Specific References (5) | bs-2684R has been referenced in 5 publications.[IF=1.42] Ruan, Wei-Min, et al. "Differential expression of glycoprotein non-metastatic melanoma protein B (GPNMB) involved in trichostatin A-induced apoptosis in gastric cancer." International journal of clinical and experimental medicine 7.12 (2014): 4857-4866. IHC-P ; Human.PubMed:25663982[IF=4.60] Abdelmagid, Samir M., et al. "Mutation in Osteoactivin Promotes RANKL-Mediated Osteoclast Differentiation and Survival, but Inhibits Osteoclast Function." Journal of Biological Chemistry (2015): jbc-M114. WB ; Mouse.PubMed:25837253[IF=3.84] Arosarena, Oneida A., et al. "Osteoactivin Promotes Migration of Oral Squamous Cell Carcinomas." Journal of Cellular Physiology (2016). WB ; Human.PubMed:26636434[IF=1.72] Frara, Nagat, et al. "Growth and repair factors, osteoactivin, matrix metalloproteinase and heat shock protein 72, increase with resolution of inflammation in musculotendinous tissues in a rat model of repetitive grasping." BMC Musculoskeletal Disorders 17.1 (2016): 1. WB ; Rat.PubMed:26781840[IF=0.00] Sondag, Gregory R. OSTEOACTIVIN AND CD44: A NOVEL INTERACTION REGULATING BONE CELL DIFFERENTIATION AND FUNCTION. Diss. Kent State University, 2015. WB ; Mouse.PubMed:000
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 免疫学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Pig, Cow, Horse, Guinea Pig,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 61kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human GPNMB:81-180/572 <Extracellular>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function:
Could be a melanogenic enzyme.
Subcellular Location:
Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Tissue Specificity:
Not restricted to the melanocytic lineage.
Similarity:
Belongs to the PMEL/NMB family.
Contains 1 PKD domain.
SWISS:
Q14956
Gene ID:
10457
Database links:Entrez Gene: 10457 Human
Entrez Gene: 93695 Mouse
Entrez Gene: 113955 Rat
Omim: 604368 Human
SwissProt: Q14956 Human
SwissProt: Q99P91 Mouse
SwissProt: Q6P7C7 Rat
Unigene: 190495 Human
Unigene: 302602 Mouse
Unigene: 13778 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:
PANC-1(Human) Cell Lysate at 30 ug
Primary: Anti-GPNMB (bs-2684R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 101 kD
Sample:
Lane1: Liver(Rat) Lysate at 30 ug
Lane2: Colon carcinoma(Human) Lysate at 30 ug
Primary: Anti-GPNMB/Osteoactivin (bs-2684R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 61kD
Observed band size : 61kD
Tissue/cell: rat lung tissue(melanoma); 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37鈩� for 20 min;
Incubation: Anti-GPNMB/Osteoactivin Polyclonal Antibody, Unconjugated(bs-2684R) 1:200, overnight at 4掳C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining