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货号： bs-2653R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-2653R
- 英文名称
- SPHK2
- 中文名称
- 鞘氨醇激酶2抗体
- 别 名
- sphingosine kinase 2; SK 2; SPK 2; C76851; MGC102297; SPHK2_HUMAN.
- Specific References (3) | bs-2653R has been referenced in 3 publications.[IF=2.71] Zhang, Ruxin, et al. "Hypoxic preconditioning protects cardiomyocytes against hypoxia/reoxygenation-induced cell apoptosis via sphingosine kinase 2 and FAK/AKT pathway." Experimental and Molecular Pathology (2015). WB ; Rat.PubMed:26621495[IF=4.29] Tran, Hai B., et al. "Cigarette smoke inhibits efferocytosis via deregulation of sphingosine kinase signaling: reversal with exogenous S1P and the S1P analogue FTY720." Journal of Leukocyte Biology (2016): jlb-3A1015. Human.PubMed:26792820[IF=3.53] Tani, Kohsuke, Keiji Tabuchi, and Akira Hara. "Hair Cell Loss Induced by Sphingosine and a Sphingosine Kinase Inhibitor in the Rat Cochlea."Neurotoxicity research 29.1 (2016): 35-46. WB, IF(ICC) ; Rat.PubMed:26472207
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 细胞生物 信号转导 细胞凋亡 激酶和磷酸酶 细胞分化
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 72 kDa
- 细胞定位
- 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human SPHK2:101-200/654
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. The gene product also enhances apoptosis in different cell types and suppresses cellular proliferation. In mast cells, the encoded protein is necessary for influx of calcium, protein kinase C activation, and cytokine production and degranulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq].
Function:
Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.
Subcellular Location:
Isoform 1: Cytoplasm. Membrane. Isoform 2: Lysosome membrane.
Similarity:
Contains 1 DAGKc domain.
SWISS:
Q9NRA0
Gene ID:
56848
Database links:Entrez Gene: 56848 Human
Entrez Gene: 56632 Mouse
Omim: 607092 Human
SwissProt: Q9NRA0 Human
SwissProt: Q9JIA7 Mouse
Unigene: 528006 Human
Unigene: 24222 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample:Stomach (Mouse) Lysate at 40 ug
Primary: Anti-SPHK2(bs-2653R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72kD
Observed band size: 75kD
Sample:Liver (Mouse) Lysate at 40 ug
Primary: Anti-SPHK2(bs-2653R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72kD
Observed band size: 75kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPHK2) Polyclonal Antibody, Unconjugated (bs-2653R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.