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货号： bs-2301R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-2301R
- 英文名称
- CD162
- 中文名称
- P选择素糖蛋白配体1抗体
- 别 名
- CD 162; CD162; CD162 antigen; CLA; Cutaneous lymphocyte associated associated antigen; P selectin glycoprotein ligand 1; P selectin glycoprotein ligand 1 precursor; P-selectin glycoprotein ligand 1; PSGL 1; PSGL-1; PSGL1; Selectin P ligand; SELPL_HUMAN; SELPLG.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.
Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.
DISEASE:
Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
Similarity:
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 8 Sushi (CCP/SCR) domains.
SWISS:
Q14242
Gene ID:
6404
Database links:Entrez Gene: 6404 Human
Entrez Gene: 363930 Rat
Omim: 600738 Human
SwissProt: Q14242 Human
Unigene: 591014 Human
Unigene: 215585 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PSGL-1广泛分布于免疫细胞表面，在生物体内可与P、L、E选择素结合。在炎症发生、发展中有重要作用.
- 产品图片
Sample:
Jurkat(Human) Cell Lysate at 30 ug
MOLT-4(Human) Cell Lysate at 30 ug
Primary: Anti-CD162 (bs-2301R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
Sample:
Raji(Human) Cell Lysate at 30 ug
U937(Human) Cell Lysate at 30 ug
Primary: Anti-CD162 (bs-2301R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 41 kD
Blank control: U937(blue).
Primary Antibody: Rabbit Anti-CD162 antibody(bs-2301R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min).Primary antibody (bs-2301R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.