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货号： bs-1925R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1925R
- 英文名称
- UCP-1
- 中文名称
- 线粒体脱偶连蛋白1抗体
- 别 名
- Uncoupling Protein-1; UCP1; UCP 1; Mitochondrial brown fat uncoupling protein 1; mitochondrial brown fat uncoupling protein; SLC25A7; Thermogenin; UCP 1; UCP; Uncoupling protein 1; UCP1_HUMAN.
- Specific References (3) | bs-1925R has been referenced in 3 publications.[IF=4.72] Lu, Xiaodan, et al. "Resistance to obesity by repression of VEGF gene expression through induction of brown-like adipocyte differentiation." Endocrinology 153.7 (2012): 3123-3132. WB, IHC-P ; Mouse.PubMed:22593269[IF=2.71] Sun, Chao, et al. "Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue." BioMed Research International 2015 (2015). WB ; Rat.PubMed:26125027[IF=2.37] Hou, Lianjie, et al. "Pig has no uncoupling protein 1." Biochemical and Biophysical Research Communications (2017). WB ;PubMed:28442343
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 免疫学 神经生物学 信号转导 线粒体
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 33kDa
- 细胞定位
- 细胞浆 线粒体
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human UCP-1:101-200/307
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat. [provided by RefSeq].
Function:
UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
Subunit:
Acts as a dimer forming a proton channel.
Subcellular Location:
Mitochondrion inner membrane; Multi-pass membrane protein.
Tissue Specificity:
Brown adipose tissue.
Similarity:
Belongs to the mitochondrial carrier family.
Contains 3 Solcar repeats.
SWISS:
P12242
Gene ID:
7350
Database links:Entrez Gene: 7350 Human
Entrez Gene: 22227 Mouse
Entrez Gene: 24860 Rat
Omim: 113730 Human
SwissProt: P25874 Human
SwissProt: P12242 Mouse
SwissProt: P04633 Rat
Unigene: 249211 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
线粒体及Cyto-c在细胞凋亡中的中心地位虽然在不同信号诱导的细胞凋亡具有一定意义，但线粒体作为细胞内死亡信号的感受者和放大者在细胞凋亡中的重要性是勿庸置疑的。
线粒体由两层膜包被,外膜平滑,内膜向内折叠形成嵴,两层膜之间有腔,线粒体中央是基质。基质内含 有与三羧酸循环所需的全部酶类,内膜上具有呼吸链酶系及ATP酶复合体。线粒体能为细胞的生命活动提供场所，是细胞内氧化磷酸化和形成ATP的主要场所。另外,线粒体有自身的DNA和遗传体系, 但线粒体基因组的基因数量有限,因此,线粒体只是一种半自主性的细胞器。
我们每时每刻都在呼吸，目的是把氧气吸入体内用于制造生物体可利用的能量分子ATP。氧气被线粒体利用制造能量的过程如同发电厂燃烧煤发电。线粒体内有两个主要部件参与能量的制造，一个部件叫做呼吸链，另一个部件叫做三磷酸腺苷酶（简称ATP酶）。顾名思义呼吸链是直接利用氧气把食物燃烧的部件，食物中储存有光合作用固化下来的太阳能，燃烧食物如同发电厂燃煤锅炉的作用，目的是把固化的太阳能释放出来推动发电机发电。ATP酶本质上是一个可以发电的分子马达，像锅炉燃煤推动发电机转动生产电流一样，固化的太阳能释放出来推动分子马达的转动可以制造能量分子ATP。我们每人每天大约消耗相当于体重数量的能量分子ATP，因此，线粒体不断制造ATP分子是维持生命活力所必需的。
线粒体是细胞有氧呼吸的主要场所.主要分为三个阶段:
第一阶段：在细胞质的基质中，一个分子的葡萄糖分解成两个分子的丙酮酸，同时脱下4个[H]酶；在葡萄糖分解的过程中释放出少量的能量，其中一部分能量用于合成ATP，产生少量的ATP。反应式：C6H12O6酶→2丙酮酸+4[H]+少量能量
第二阶段：丙酮酸进入线粒体的基质中，两分子丙酮酸和6个水分子中的氢全部脱下，共脱下20个[H]，丙酮被氧化分解成二氧化碳；在此过程释放少量的能量，其中一部分用于合成ATP，产生少量的能量。反应式：2丙酮酸+6H2O酶→20[H]+6CO2+少量能量
第三阶段：在线粒体的内膜上，前两阶段脱下的共24个[H]与从外界吸收或叶绿体光合作用产生的6个O2结合成水；在此过程中释放大量的能量，其中一部分能量用于合成ATP，产生大量的能量。反应式：24[H]+6O2酶→12H2O+大量能量。
- 产品图片
Sample:
Thymus (Mouse) Lysate at 40 ug
Primary: Anti- UCP-1 (bs-1925R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Positive: Para-cancer tissue;
Negative: Human gastric carcinoma;
Paraformaldehyde-fixed, paraffin embedded; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (UCP-1) Polyclonal Antibody, Unconjugated (bs-1925R) at 1:400 overnight at 4°C, followed by a conjugated secondary (bs-0295G-Cy3) at [1:500] for 90 minutes and DAPI staining of the nuclei.
Blank control: Mouse spleen (blue).
Primary Antibody:Rabbit Anti-UCP-1 antibody(bs-1925R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (bs-1925R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.