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货号： bs-0634R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0634R
- 英文名称
- Aquaporin 4
- 中文名称
- 水通道蛋白4抗体
- 别 名
- aquaporin 4; WCH4; Mercurial-insensitive water channel; MIWC; AQP 4; AQP4; Aquaporin4; HMIWC 2; HMIWC2; Mercurial insensitive water channel; MGC22454; MIWC; AQP4_HUMAN; Aquaporin-4; AQP-4.
- Specific References (4) | bs-0634R has been referenced in 4 publications.[IF=2.51] Gang, Zhang, et al. "Detection of hypoxic-ischemic brain injury with 3D-enhanced T2* weighted angiography (ESWAN) imaging." European Journal of Radiology (2013). IHC-P ; Pig.PubMed:23777745[IF=2.02] Duan, Hubin, et al. "The role of neuropeptide Y and aquaporin 4 in the pathogenesis of intestinal dysfunction caused by traumatic brain injury." Journal of Surgical Research (2013). IHC-P ; Rat.PubMed:23622727[IF=0.23] Li, Cheng, et al. "Melatonin lowers edema after spinal cord injury." Neural Regeneration Research 9.24 (2014): 2205. IHC-P ; Rat.PubMed:25657743[IF=0.64] Rajesh, Kumar, Li Xiaojie, and Kong Xiangying. "The Effect of Early Intervention and Rehabilitation in the Expression of Aquaporin-4; and Ultrastructure Changes on Rats Offsprings Damaged Brain Caused by Intrauterine Infection." Journal of Korean Neurosurgical Society 58.1 (2015): 14-21. WB ; Rat.PubMed:26279808
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 信号转导 通道蛋白 新陈代谢
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Pig, Cow, Rabbit, Sheep,
- 产品应用
- WB=1:500-2000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 36kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human AQP4:271-323/323 <Cytoplasmic>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function:
Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
Subunit:
Homotetramer. Part of a complex containing MLC1, TRPV4, HEPACAM and ATP1B1.
Subcellular Location:
Membrane; Multi-pass membrane protein.
Tissue Specificity:
Brain, muscle, heart, kidney, lung, and trachea.
Similarity:
Belongs to the MIP/aquaporin (TC 1.A.8) family.
SWISS:
P55088
Gene ID:
361
Database links:Entrez Gene: 281008Cow
Entrez Gene: 361Human
Entrez Gene: 11829Mouse
Entrez Gene: 25293Rat
Omim: 600308Human
SwissProt: O77750Cow
SwissProt: P55087Human
SwissProt: P55088Mouse
SwissProt: P47863Rat
Unigene: 281008Cow
Unigene: 315369Human
Unigene: 250786Mouse
Unigene: 90091Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
通道蛋白（Channel Protein）
水通道蛋白(AQPs)广泛分布于机体不同组织器官中,脑组织中的水通道蛋白主要为AQP4.
AQP-4蛋白主要在星型胶质细胞和室管膜细胞内表达,尤其在于毛细血管和软脑膜直接接触的胶质细胞上及血管周表达丰富.
水通道蛋白4的分布特点与其功能密切相关,可能是脑脊液重吸收、渗透压调节、脑水肿形成等生理、病理过程的分子生物学基础.研究水通道蛋白4可为脑水肿及水代谢疾病提供分子水平的理论依据,同时为临床治疗脑水肿及水代谢疾病提供一种新途径.
- 产品图片
Sample: Muscle(Mouse) lysate at 30ug; Primary: Anti-Aquaporin 4 (bs-0634R) at 1:300; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size:36kDObserved band size:35kD
Sample: mouse lung lysate at 30ug;
Primary: Anti-Aquaporin 4 (bs-0634R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bse-0295G-HRP) at 1: 5000;
Predicted band size:36 kD
Observed band size:34 kD
Tissue/cell: human laryngocarcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated(bs-0634R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated(bs-0634R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AQP4 Polyclonal Antibody, Unconjugated(bs-0634R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: K562 (blue).
Primary Antibody:Rabbit Anti-Aquaporin 4 antibody (bs-0634R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions;
Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (bs-0634R, 1μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min at room temperature. Acquisition of 20,000 events was performed.