产品中心
当前位置：首页>产品中心Anti-alpha Elastin/Tropoelastin
货号： bs-1756R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1756R
- 英文名称
- alpha Elastin/Tropoelastin
- 中文名称
- α弹性蛋白抗体
- 别 名
- Elastin alpha; Elastin; Elastin isoform a; ELN; ELN_HUMAN; FLJ38671; FLJ43523; Supravalvular aortic stenosis; SVAS; Tropoelastin; WBS; Williams Beuren syndrome; Williams syndrome region; WS.
- Specific References (3) | bs-1756R has been referenced in 3 publications.[IF=6.87] Konii, Hanae, et al. "Stimulatory Effects of Cardiotrophin 1 on Atherosclerosis."Hypertension?(2013): HYPERTENSIONAHA-113 WB ; Human.PubMed:24041953[IF=3.55] Guo, Hong-Feng, et al. "Piezoelectric PU/PVDF electrospun scaffolds for wound healing applications." Colloids and Surfaces B: Biointerfaces (2012). WB ; Mouse.PubMed:22503631[IF=3.53] Hasegawa A, Sato K, Shirai R, Watanabe R, Yamamoto K, et al. (2014) Vasoprotective Effects of Urocortin 1 against Atherosclerosis In Vitro and In Vivo. PLoS ONE 9(12): e110866 WB ; Human.PubMed:5462164
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 免疫学 神经生物学 转录调节因子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 70kDa
- 细胞定位
- 细胞外基质 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Elastin:681-786/786
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Elastin is a major structural protein of tissues such as aorta, which must expand rapidly and recover completely. Elastin chains are cross-linked together into an extensible 3D network. It is a molecular determinant of late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle. Elastin forms a complex with BGN and MFAP2.
Function:
Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
Subunit:
The polymeric elastin chains are cross-linked together into an extensible 3D network. Forms a ternary complex with BGN and MFAP2. Interacts with MFAP2 via divalent cations (calcium > magnesium > manganese) in a dose-dependent and saturating manner.
Subcellular Location:
Secreted, extracellular space, extracellular matrix. Note=Extracellular matrix of elastic fibers.
Tissue Specificity:
Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
DISEASE:
Defects in ELN are the cause of cutis laxa, autosomal dominant, type 1 (ADCL1) . A connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. Face, hands, feet, joints, and torso may be differentially affected. Additional variable clinical features are gastrointestinal diverticula, hernia, and genital prolapse. Rare manifestations are pulmonary artery stenosis, aortic aneurysm, bronchiectasis, and emphysema.
Similarity:
Belongs to the elastin family.
SWISS:
P15502
Gene ID:
2006
Database links:Entrez Gene: 2006 Human
Entrez Gene: 13717 Mouse
Omim: 130160 Human
SwissProt: P15502 Human
SwissProt: P54320 Mouse
Unigene: 647061 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
- 产品图片
Sample: Lung (Mouse) Lysate at 30 ug
Primary: Anti- alpha Elastin/Tropoelastin (bs-1756R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
Predicted band size: 70 kD
Observed band size: 68 kD
Images provided the Independent Validation Program (badge number 28751): Formalin-fixed and paraffin embedded mouse heart labeled with Rabbit Anti-alpha Elastin Polyclonal Antibody (bs-1756R) at 1:1000 4 ∑C temperature overnight 4 ∑C followed by conjugation to secondary antibody.
The figure annotation: The blue histogram is unstained cells.The green histogram is cells stained with Rabbit Anti-alpha Elastin/Tropoelastin antibody (bs-1756R)plus secondary antibody.
Controls: Positive control: A549 cells .Isotype control: Cell lines treated with rabbit IgG (bs-0295P)instead of the primary antibody to confirm that primary antibody binding is specific.Secondary only control: Both cell lines treated with Goat Anti-rabbit IgG/FITC antibody (bs-0295G-FITC) to confirm no background signal produced from secondary antibody alone. 1ug in 100uL 1 X PBS containing 0.5% BSA.