Anti-ox-LDL【科瑞奥博】

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货号： bs-1698R 基本售价： 780.0 元规格： 50ul

别名: ox-LDL: LDL (Copper oxidized); Cu2SO4 oxidized low density lipoprotein; oxidized low density lipoprotein; Low density lipoprotein; MDA oxidized LDL; MDA oxidized low density lipoprotein. 氧化型低密度脂蛋白；

: Specific References  (2)     |     bs-1698R has been referenced in 2 publications.
[IF=4.39] Wen, Song, et al. "OxLDL-targeted iron oxide nanoparticles for in vivo MRI detection of perivascular carotid collar induced atherosclerotic lesions in ApoE-deficient mice." Journal of Lipid Research 53.5 (2012): 829-838.  IHC-F ;  Mouse.
PubMed:22393161
[IF=20.06] Martinez, Jennifer, et al. "Molecular characterization of LC3-associated phagocytosis reveals distinct roles for Rubicon, NOX2 and autophagy proteins." Nature Cell Biology (2015).  Mouse.
PubMed:26098576

产品应用: WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3ug/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品介绍: background:
Low-density lipoprotein (LDL) is the carrier protein for cholesterol in the blood. LDL binds to its receptor on the capillary walls and thereby mediates the uptake and clearence of cholesterol from the circulation. In atherosclerotic lesions oxidatively modified LDL is found and oxidized LDL is specifically recognized and ingested by macrophages via scavenger receptor A and CD36. Oxidized LDL may be a marker of atherosclerosis but the precise changes in oxidized LDL are not well described. Low-density lipoprotein oxidised with Cu2SO4.
When too much LDL cholesterol circulates in the blood, it can slowly build up in the inner walls of the arteries that feed the heart and brain. Together with other substances it can form plaque, a thick, hard deposit that can clog those arteries. This condition is known as atherosclerosis. Oxidized lipoproteins are formed by free radical damage to lipids that accumulate in macrophages and smooth muscle cells causing foam cell formation, an initial step in the disease.

Subcellular Location:
Secreted (probable)

SWISS:
N/A

Gene ID:
N/A

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

氧化低密度脂蛋白是低密度脂蛋白(low density lipoprotein,LDL)经氧化修饰后形成的,经研究认为:OXLDL的增高与动脉粥样硬化性心血管病(Arteriosclerosis cardiovascular disease,ASCVD)的发生有密切关系。

产品图片: Sample:
Hcclm3(Human) Cell Lysate at 30 ug
Primary: Anti-ox-LDL (bs-1698R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ox-LDL) Polyclonal Antibody, Unconjugated (bs-1698R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-ox-LDL antibody (bs-1698R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.