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货号： bs-1573R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1573R
- 英文名称
- BBC3/PUMA
- 中文名称
- p53正向细胞凋亡调控因子抗体
- 别 名
- PUMA; bcl-2 binding component 3; BBC 3; BBC3; BCL 2 binding component 3; BCL2 binding component 3; JFY 1; JFY1; p53 up regulated modulator of apoptosis; p53 Upregulated Modulator of Apoptosis; PUMA alpha; PUMA/JFY1; BBC3_RAT.
- Specific References (2) | bs-1573R has been referenced in 2 publications.[IF=1.96] Zhang, Pei, et al. "Epithelial-mesenchymal transition is necessary for acquired resistance to cisplatin and increases the metastatic potential of nasopharyngeal carcinoma cells." International Journal of Molecular Medicine (2013). WB ; Human.PubMed:24173500[IF=5.62] Qiu, Shi, et al. "A small peptide derived from p53 linker region can resume the apoptotic activity of p53 by sequestering iASPP with p53." Cancer Letters 356.2 (2015): 910-917. IP ; Human.PubMed:25444901
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 免疫学 细胞凋亡 线粒体
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 21kDa
- 细胞定位
- 细胞浆 线粒体
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human BBC3:131-180/193
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]
Function:
Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Essential for ER stress-induced cell death.
Subunit:
Interacts with BCL2, BCL2L1/BCL-XL, MCL1 and BCL2A1.
Subcellular Location:
Mitochondrion. Note=Localized to the mitochondria in order to induce cytochrome c release.
Similarity:
Belongs to the Bcl-2 family.
SWISS:
Q96PG8
Gene ID:
27113
Database links:Entrez Gene: 27113 Human
Entrez Gene: 170770 Mouse
Entrez Gene: 317673 Rat
Omim: 605854 Human
SwissProt: Q9BXH1 Human
SwissProt: Q99ML1 Mouse
SwissProt: Q80ZG6 Rat
Unigene: 467020 Human
Unigene: 7660 Mouse
Unigene: 25176 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PUMA在由p53诱导而经历细胞凋亡的细胞中被p53活化。在线粒体，PUMA 诱导细胞色素C的释放和激活对细胞凋亡的快速诱导。PUMA 与Bcl2、Bcl-XL、类Bcl2-1蛋白相互作用，促使线粒体易位和BAX的多聚化。PUMA在通过依赖细胞色素C/APAF1 途径介导p53诱导的细胞死亡中也发挥作用。
- 产品图片
Sample: K562 Cell Lysate at 30 ug
Primary: Anti- BBC3 (bs-1573R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 25 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BBC3/PUMA Polyclonal Antibody, Unconjugated(bs-1573R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: U-87MG(blue).
Primary Antibody:Rabbit Anti-BBC3 PUMA antibody(bs-1573R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (bs-1573R,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.