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货号： bs-2216R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-2216R
- 英文名称
- IL-1RA
- 中文名称
- 白介素-1受体拮抗剂抗体
- 别 名
- Interleukin-1 receptor antagonist; ICIL 1RA; IL1 inhibitor; ICIL1RA; IL1F3; IL1RN; IRAP; IL1RA_HUMAN.
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 免疫学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Sheep,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 17kDa
- 细胞定位
- 细胞浆 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human IL-1RA:26-120/178
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
IL1RA is a member of the Interleukin 1 cytokine family. This protein inhibits the activities of Interleukin 1, alpha (IL1A) and Interleukin 1, beta (IL1B), and modulates a variety of Interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
Function:
Inhibits the activity of interleukin-1 by binding to eceptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure.
Subcellular Location:
Isoform 1: Secreted.
Isoform 2, 3, 4: Cytoplasm.
Tissue Specificity:
The intracellular form of IL1RN is predominantly expressed in epithelial cells.
DISEASE:
Microvascular complications of diabetes 4 (MVCD4) [MIM:612628]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Interleukin 1 receptor antagonist deficiency (DIRA) [MIM:612852]: A rare autoinflammatory disease of skin and bone resulting in sterile multifocal osteomyelitis, periostitis, and pustulosis from birth. The term autoinflammatory disease describes a group of disorders characterized by attacks of seemingly unprovoked inflammation without significant levels of autoantibodies and autoreactive T-cells. Note=The disease is caused by mutations affecting the gene represented in this entry.
SWISS:
P25085
Gene ID:
3557
Database links:Entrez Gene: 3557 Human
Entrez Gene: 16181 Mouse
Entrez Gene: 60582 Rat
Omim: 147679 Human
SwissProt: P18510 Human
SwissProt: P25085 Mouse
SwissProt: P25086 Rat
Unigene: 81134 Human
Unigene: 882 Mouse
Unigene: 162640 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
IL-1受体拮抗剂(IL-1ra)是IL-1强有力的内生拮抗剂，通过与白细胞介素-1的Ⅰ型和Ⅱ型受体结合，特异性地阻断白细胞介素-1的生物学作用。白介素1受体拮抗剂(IL-1Ra)是体内天然的一种对IL-1有拮抗作用的细胞因子。
- 产品图片
Sample: Lane1:Plasma(Mouse) Tissue Lysate at 30 ug
Lane2:Hemocyte(Mouse) Tissue Lysate at 30 ug
Primary: Anti-IL-1RA (bs-2216R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 23 kD
Sample: Spleen(Mouse) Tissue Lysate at 30 ug
Primary: Anti-IL-1RA (bs-2216R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kD
Observed band size: 23 kD
Tissue/cell: Human oral squamous cell carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IL-1RA Polyclonal Antibody, Unconjugated(bs-2216R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining