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货号： bs-1352R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1352R
- 英文名称
- MCL1
- 中文名称
- 髓样细胞白血病-1抗体
- 别 名
- myeloid cell leukemia 1; myeloid cell leukemia sequence 1; MCL-1; MCL1L; MCL 1; mcl1/EAT; MGC104264; MGC1839; TM; MCL1S; EAT) Bcl 2 related protein EAT/mcl1; BCL2 related; BCL2L3; EAT; Induced myeloid leukemia cell differentiation protein Mcl 1; myeloid cell leukemia sequence 1; Myeloid cell leukemia sequence 1 BCL2 related; Myeloid cell leukemia sequence 1 isoform 1; OTTHUMP00000032794; OTTHUMP00000032795; TM; bcl2-L-3; BCL2L3; EAT; Mcl-1; MCL1-ES; mcl1/EAT.
- Specific References (4) | bs-1352R has been referenced in 4 publications.[IF=3.73] Chen, Xin-mei, et al. "Wogonin Has Multiple Anti-Cancer Effects by Regulating c-Myc/SKP2/Fbw7α and HDAC1/HDAC2 Pathways and Inducing Apoptosis in Human Lung Adenocarcinoma Cell Line A549." PLOS ONE 8.11 (2013) WB ; Human.PubMed:24265759[IF=1.96] Zhang, Pei, et al. "Epithelial-mesenchymal transition is necessary for acquired resistance to cisplatin and increases the metastatic potential of nasopharyngeal carcinoma cells." International journal of molecular medicine (2013). WB ; Human.PubMed:24173500[IF=1.48] Chen, Xin‑Mei, et al. "Ampelopsin induces apoptosis by regulating multiple c‑Myc/S‑phase kinase‑associated protein 2/F‑box and WD repeat‑containing protein 7/histone deacetylase 2 pathways in human lung adenocarcinoma cells." Molecular Medicine Reports. WB ; Human.PubMed:25333250[IF=1.44] Wang, Fei-yu, et al. "A small hairpin RNA targeting myeloid cell leukemia-1 enhances apoptosis in host macrophages infected with Mycobacterium tuberculosis." Journal of Microbiology 54.4 (2016): 330-337. Mouse.PubMed:27033209
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 免疫学 信号转导 细胞凋亡 转录调节因子 线粒体
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Rabbit, Sheep,
- 产品应用
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1µg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 39kDa
- 细胞定位
- 细胞核 细胞浆 细胞膜 线粒体
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human Mcl-1:201-350/350
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Mcl1 is an anti-apoptotic member of Bcl2 family originally isolated from the ML1 human myeloid leukemia cell line during phorbol ester-induced differentiation along the monocyte/macrophage pathway. Mcl1 localizes to the mitochondria, interacts with and antagonizes pro-apoptotic Bcl2 family members, and inhibits apoptosis by a number of cytotoxic stimuli. It is involved in programing of differentiation and concomitant maintenance of viability but not of proliferation. Isoform 1 inhibits apoptosis while isoform 2 promotes it. Expression increases early during phorbol-ester induced differentiation along the monocyte/macrophage pathway in myeloid leukemia cell lines ML1.
Function:
Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.
Subunit:
Interacts with BAD, BOK, BIK and BFM (By similarity). Interacts with PMAIP1. Isoform 1 interacts with BAX, BAK1, TPT1 and BCL2L11. Heterodimer of isoform 1 and isoform 2. Homodimers of isoform 1 or isoform 2 are not detected. Isoform 2 does not interact with pro-apoptotic BCL2-related proteins.
Subcellular Location:
Membrane; Single-pass membrane protein (Potential). Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Note=Cytoplasmic, associated with mitochondria.
Post-translational modifications:
Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.
Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.
Phosphorylated on Thr-163. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.
Similarity:
Belongs to the Bcl-2 family.
SWISS:
Q07820
Gene ID:
4170
Database links:Entrez Gene: 4170 Human
Entrez Gene: 17210 Mouse
Entrez Gene: 60430 Rat
Omim: 159552 Human
SwissProt: Q07820 Human
SwissProt: P97287 Mouse
SwissProt: Q9Z1P3 Rat
Unigene: 632486 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Mcl1蛋白是bcl-2家族成员,它在调控肿瘤细胞的凋亡、增殖、分化等过程中起一定作用。
- 产品图片
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BCL2L3) Polyclonal Antibody, Unconjugated (bs-1352R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Mcl1/BCL2L3 Polyclonal Antibody, Unconjugated(bs-1352R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line): MCF7 (blue).
Primary Antibody (green line): Rabbit Anti-MCL1 antibody(bs-1352R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): F(ab’)2 fragment goat anti-rabbit IgG-FITC.
Dilution: 1μg /test.
Protocol
The cells were fixed with 2% paraformaldehyde for 10 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.