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货号： bs-0931R 基本售价： 1580.0 元 规格： 100ul
产品信息
- 产品编号
- bs-0931R
- 英文名称
- Histone H1.4 (Acetyl K53)
- 中文名称
- 乙酰化组蛋白H1b抗体
- 别 名
- Histone H1.4(Ac-Lys53); Histone H1b (Ac-Lys53); Acetyl-Histone H1.4 (Lys53); Acetyl-Histone H1.4 (K53); HIST1H1E; H1F4; Histone H1.4; Histone H1b; H14_HUMAN; Histone H1s-4; H1 histone family member 4; H1E; Hist1h1e; Histone 1 H1e; Histone cluster 1 H1e; Histone H1; MGC116819.
- 规格价格
- 100ul/1580元购买 大包装/询价
- 说 明 书
- 100ul
- 产品类型
- 乙酰化抗体
- 研究领域
- 肿瘤 发育生物学 信号转导 细胞凋亡 转录调节因子 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Pig, Cow, Horse,
- 产品应用
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 24kDa
- 细胞定位
- 细胞核
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated Synthesised acetylpeptide derived from human Histone H1b around the acetylation site of Lys53:AS(Ac-K)ER
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Histone H1b are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Function:
Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.
Subcellular Location:
Nucleus. Chromosome.
Post-translational modifications:
Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1.
Similarity:
Belongs to the histone H1/H5 family.
Contains 1 H15 (linker histone H1/H5 globular) domain.
SWISS:
P10412
Gene ID:
3008
Database links:Entrez Gene: 3008 Human
Omim: 142220 Human
SwissProt: P10412 Human
Unigene: 248133 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
转录调节因子（Transcriptin Regulators）
- 产品图片
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H1.4 (Acetyl K53)) Polyclonal Antibody, Unconjugated (bs-0931R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H1.4 (Acetyl K53)) Polyclonal Antibody, Unconjugated (bs-0931R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Molt-4 cells were fixed with 4% PFA for 10min at room temperature ,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Histone H1.4 (Acetyl K53) Antibody(bs-0931R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).