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货号： bs-1318R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1318R
- 英文名称
- IL-5
- 中文名称
- 白细胞介素5抗体
- 别 名
- B Cell Differentiation Factor I; IL5; IL 5; Colony stimulating factor; Colony Stimulating Factor Eosinophil; EDF; Eosinophil; Eosinophil Differentiation Factor; IL 5; Interleukin 5; B-cell differentiation factor I; IL-5; IL5_RAT; Interleukin-5; T Cell Replacing Factor; T-cell replacing factor; Interleukin5; TRF.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 免疫学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 13kDa
- 细胞定位
- 分泌型蛋白
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from mouse IL-5:56-132/132
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Interleukin 5 is a cytokine produced primarily by activated T lymphocytes. It exists as an antiparallel disulfide linked homodimeric glycoprotein with 115 amino acid residues in each chain. Known also as EDF (eosinophil differentiating factor), functions predominantly as an eosinophilopoietic factor. Analysis of its crystal structure reveals a novel two domain structure, with each domain showing significant homology to the cytokine fold in GMCSF, MCSF, IL2, IL4 and growth hormone. Human and mouse IL5 have 70% amino acid sequence homology.
Function:
Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.
Subunit:
Homodimer; disulfide-linked.
Subcellular Location:
Secreted.
Similarity:
Belongs to the IL-5 family.
SWISS:
P04401
Gene ID:
16191
Database links:Entrez Gene: 3567 Human
Omim: 147850 Human
SwissProt: P05113 Human
Unigene: 2247 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
白细胞介素-5（IL-5）的表达对嗜酸粒细胞（EOS）有浸润聚集的作用，目前多用变态反应方面的研究。
支气管哮喘的重要特征主要是慢性非特异性炎症改变，其病因与发病机制十分复杂，白介素5在支气管哮喘发病机理上，近年来受到了很大关注。
- 产品图片
Sample:
Lovo Cell (Human) Lysate at 30 ug
Primary: Anti- IL-5 (bs-1318R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13kD
Observed band size: 10kD
Paraformaldehyde-fixed, paraffin embedded (Human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-5) Polyclonal Antibody, Unconjugated (bs-1318R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-5) Polyclonal Antibody, Unconjugated (bs-1318R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.