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货号： bs-20116R-AP 基本售价： 2980.0 元 规格： 100ul
产品信息
- 产品编号
- bs-20116R-AP
- 英文名称
- Anti-CSFV E2/AP
- 中文名称
- 碱性磷酸酶（AP）标记的猪瘟病毒E2抗体
- 别 名
- CSFV Polyprotein; CSFV E2 protein; CSFV E2; polyprotein [Classical swine fever virus]; polyprotein; CSFV polyprotein; E2; Envelope glycoprotein E2; CSFV Envelope glycoprotein E2; Classical swine fever virus E2; Classical swine fever virus Envelope glycoprotein E2; structural protein E2 [Classical swine fever virus].
- 规格价格
- 100ul/2980元购买 大包装/询价
- 说 明 书
- 100ul
- 研究领域
- 免疫学 细菌及病毒
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- CSFV
- 产品应用
- WB=1:50-200 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 42kDa
- 性 状
- Lyophilized or Liquid
- 浓 度
- 2mg/1ml
- 免 疫 原
- CSFV E2 protein
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- 产品介绍
- background:
Classical swine fever(CSF) is a kind of acute and intense contagious disease in swine, it material damages swine industry. CSF could cause swine appearing disseminated hemorrhage in systemic organ and tissue, degeneration and necrosis in small vascular and capillary endothelial cells. Classical swine fever virus (CSFV) belongs to genera Pestivirus, family Flaviviridae, it’s genome total length is 12.3Kb,including a big ORF, encoding 11 structural and nonstructural proteins.
Function:
E(rns), E1 and E2 are responsible of cell attachment and subsequent fusion of viral and cellular membrane.
P7 forms a leader sequence to properly orient NS2 in the membrane.
Uncleaved NS2-3 is required for production of infectious virus.
NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus.
NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase.
NS4A is a cofactor for the NS3 protease activity.
RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome.
Subunit:
The E(rns) glycoprotein is found as a homodimer; disulfide-linked. The E1 and E2 envelope glycoproteins form disulfide-linked homodimers as well as heterodimers.
Subcellular Location:
Host cell surface.
Post-translational modifications:
The E(rns) glycoprotein is heavily glycosylated.
The viral RNA of pestiviruses is expressed as a single polyprotein which undergoes post-translational proteolytic processing resulting in the production of at least eleven individual proteins. The N-terminal protease cleaves itself from the nascent polyprotein autocatalytically and thereby generates the N-terminus of the adjacent viral capsid protein C.
Cleavage between E2 and p7 is partial.
Similarity:
Belongs to the pestivirus polyprotein family.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 peptidase C53 domain.
Contains 1 peptidase C74 domain.
Contains 1 peptidase S31 domain.
Contains 1 RdRp catalytic domain.
Database links:Entrez Gene: 920146 CSFVA
SwissProt: P19712 CSFVA
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
猪瘟(Classical Swine Fever,CSF)是由猪瘟病毒(CSFV)引起的猪急性高度接触性传染性疾病,是严重威胁养猪业,具有重要经济意义的病毒性疾病之一,猪瘟可引起猪的全身组织器官弥漫性出血,小血管和毛细血管内皮细胞发生变性、坏死。
猪瘟病毒为黄病毒科(Flaviviridae)瘟病毒属(Pestivirus)成员,基因组大小约12.3kb,含一个大的开放阅读框(ORF),由11 个编码结构蛋白与非结构蛋白的基因组成。猪瘟病毒对血管组织具有亲嗜性,病理组织学检查时发现,组织器官的出血斑点主要是由于小血管和毛细血管内皮细胞发生肿胀、变性和坏死;梗死灶的发生主要是由于小动脉管内皮细胞发生变性、坏死、剥脱后,血管内膜粗糙,官腔内血栓形成,导致官腔狭窄或闭塞所致。此外,还可见到心肌呈实质变性,但病猪的肾脏等器官的实质细胞没有变性、坏死等病理变化。