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货号： bs-1279R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1279R
- 英文名称
- HLA E
- 中文名称
- 人类白细胞抗原E抗体
- 别 名
- HLA class I histocompatibility antigen E alpha chain; DKFZp686P19218; EA1.2; EA2.1; HLA 6.2; HLA class I histocompatibility antigen alpha chain E; HLA class I histocompatibility antigen E alpha chain precursor; HLA-6.2; HLA6.2; HLAE; Lymphocyte antigen; Major histocompatibility complex class I E; MHC; MHC class I antigen E; MHC HLA E alpha 1; MHC HLA E alpha 2.1;HLA-E; QA1.
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 免疫学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-F=1:400-800 Flow-Cyt=3ug/test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 37kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human HLA E:251-358/358 <Cytoplasmic>
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. [provided by RefSeq, Jul 2008]
Function:
Preferably binds to a peptide derived from the signal sequence of most HLA-A, -B, -C and -G molecules.
Subunit:
Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin).
Subcellular Location:
Membrane; Single-pass type I membrane protein.
Similarity:
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
SWISS:
P13747
Gene ID:
3133
Database links:Entrez Gene: 3133 Human
Omim: 143010 Human
SwissProt: P13747 Human
Unigene: 650174 Human
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
人类白细胞抗原E(Human Leukocyte Antigen-E )是一种非典型的白细胞抗原Ⅰ族组织相溶性抗原,在病理情况下HLA-G只在某些癌细胞表达,HLA-E是一个具有高度肿瘤特异性的肿瘤标志物。
- 产品图片
Sample:
Raji Cell (Human) Lysate at 30 ug
Primary: Anti-HLA E (bs- 1279R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD
Sample:
K562 Cell (Human) Lysate at 30 ug
Primary: Anti-HLA E (bs- 1279R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37 kD
Observed band size: 35 kD
Blank control: A549.
Primary Antibody (green line): Rabbit Anti-HLAE antibody (bs-1279R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.