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货号： bs-1214R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1214R
- 英文名称
- TRAIL
- 中文名称
- 肿瘤坏死因子相关凋亡诱导配体抗体
- 别 名
- APO2L; TNFSF10; Apo 2 ligand; Apo 2L; CD 253; CD253; CD253 antigen; Protein TRAIL; TL 2; TL2; TNF Related Apoptosis Inducing Ligand; TNF related apoptosis inducing ligand TRAIL; TNFSF 10; TNFSF10 protein; TRAIL; TRAIL PEN; Tumor Necrosis Factor (ligand) Superfamily Member 10; TNF10_HUMAN.
- Specific References (1) | bs-1214R has been referenced in 1 publications.[IF=3.53] Fang C, Zhang J, Qi D, Fan X, Luo J, et al. (2014) Evodiamine Induces G2/M Arrest and Apoptosis via Mitochondrial and Endoplasmic Reticulum Pathways in H446 and H1688 Human Small-Cell Lung Cancer Cells. PLoS ONE 9(12): e115204. WB ; Human.PubMed:25506932
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 神经生物学 信号转导 细胞凋亡
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 31kDa
- 细胞定位
- 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human TRAIL:185-281/281
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. This protein binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of this protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3.(tumor necrosis factor-related apoptosis-inducing ligand)
Function:
Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
Subunit:
Homotrimer.
Subcellular Location:
Membrane; Single-pass type II membrane protein (Potential).
Tissue Specificity:
Widespread; most predominant in spleen, lung and prostate.
Similarity:
Belongs to the tumor necrosis factor family.
SWISS:
P50591
Gene ID:
8743
Database links:Entrez Gene: 8743 Human
Entrez Gene: 22035 Mouse
Entrez Gene: 246775 Rat
Omim: 603598 Human
SwissProt: P50591 Human
SwissProt: P50592 Mouse
Unigene: 478275 Human
Unigene: 1062 Mouse
Unigene: 83627 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
肿瘤坏死因子相关的凋亡诱导配体(TNF related apoptosis inducingligand，TRAIL)又称凋亡素2配体(Apo2 ligand，Apo2L)或死亡配体，属于肿瘤坏死因子家族，能选择性的诱导肿瘤细胞凋亡，而正常细胞则可以逃逸他的杀伤作用，有望成为一种新的抗肿瘤方法。
- 产品图片
Sample:
Lung (Mouse) Lysate at 30 ug
U251 Cell Lysate at 30 ug
Primary: rabbit Anti- TRAIL (bs-1214R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1:5000 dilution;
Predicted band size: 31 kD
Observed band size: 26 kD
Tissue/cell: mouse spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TRAIL Polyclonal Antibody, Unconjugated(bs-1214R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-TRAIL Polyclonal Antibody, Unconjugated(bs-1214R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining