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货号： bs-1205R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1205R
- 英文名称
- ROCK2
- 中文名称
- Rho相关蛋白激酶2抗体
- 别 名
- p164 ROCK 2; p164 ROCK-2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha; coiled-coil-containing protein kinase 2; KIAA0619; Rho associated, coiled coil containing protein kinase II; Rho-associated; Rho-associated protein kinase 2; Rock2; ROCK2_HUMAN.
- Specific References (2) | bs-1205R has been referenced in 2 publications.[IF=4.15] Wang, Nan, et al. "Vascular endothelial growth factor stimulates endothelial differentiation from mesenchymal stem cells via Rho/myocardin-related transcription factor-A signaling pathway." The International Journal of Biochemistry & Cell Biology (2013). WB ; Rat.PubMed:23624342[IF=4.19] He, Hongpeng, et al. "Transcription of HOTAIR is regulated by RhoC-MRTF-A-SRF signaling pathway in human breast cancer cells." Cellular Signalling (2017). WB ; Human.PubMed:28069441
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 细胞生物 信号转导 细胞凋亡 激酶和磷酸酶
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Rabbit,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 161kDa
- 细胞定位
- 细胞浆 细胞膜
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human ROCK2:1001-1300/1388
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008].
Function:
Regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction.
Subcellular Location:
Cytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane.
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.Phosphorylation at Tyr-722 reduces its binding to RHOA and iscrucial for focal adhesion dynamics. Dephosphorylation by PTPN11stimulates its RHOA binding activity.
Cleaved by granzyme B during apoptosis. This leads toconstitutive activation of the kinase and membrane blebbing.
Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thrprotein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 REM (Hr1) repeat.
SWISS:
O75116
Gene ID:
9475
Database links:Entrez Gene: 9475 Human
Entrez Gene: 19878 Mouse
Entrez Gene: 25537 Rat
Omim: 604002 Human
SwissProt: O75116 Human
SwissProt: P70336 Mouse
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
ROCK2（Rho kinase alpha）是ROCK1的同工酶。是一个丝/苏氨基酸激酶，可调节细胞因子，平滑肌收缩和肌动蛋白的形成。
- 产品图片
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-ROCK2 (bs-1205R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 161 kD
Observed band size: 161 kD
Sample:
Lane1: Brain (Rat) Lysate at 30 ug
Lane2: Colon carcinoma (Human) Lysate at 30 ug
Primary: Anti-ROCK2 (bs-1205R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 161kD
Observed band size : 161kD
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ROCK2 Polyclonal Antibody, Unconjugated(bs-1205R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining