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货号： bs-1201R 基本售价： 1380.0 元 规格： 100ul
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1201R
- 英文名称
- PAX8
- 中文名称
- 配对盒基因8抗体
- 别 名
- Paired Box Gene 8; paired box homeotic gene 8; Paired box protein Pax 8; Paired Domain Gene 8; PAX 8;PAX-8; PAX8_HUMAN.
- Specific References (1) | bs-1201R has been referenced in 1 publications.[IF=1.38] Vadasz, Stephanie, et al. "Second and third trimester amniotic fluid mesenchymal stem cells can repopulate a de-cellularized lung scaffold and express lung markers." Journal of Pediatric Surgery (2014). Human.PubMed:25475793
- 规格价格
- 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 100ul 200ul
- 研究领域
- 肿瘤 发育生物学 信号转导 转录调节因子
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat, Dog, Cow,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 48kDa
- 细胞定位
- 细胞核
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from human PAX8:21-130/457
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
This protein is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain, an octapeptide, and a paired-type homeodomain. This family plays critical roles during fetal development and cancer growth. The specific function of the PAX8 is unknown but it may involve kidney cell differentiation, thyroid development, or thyroid dysgenesis. Alternative splicing in the gene by inclusion or exclusion of exons 7 and/or 8 has produced several known products but the biological significance of the variants is unknown. Several other splice variants have been proposed but the full nature of these products has not been described. Pax8 is also a marker of otic progenitor cells.
Function:
Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells.
Subunit:
Interacts with WWTR1.
Subcellular Location:
Nucleus.
Tissue Specificity:
Expressed in the excretory system, thyroid gland and Wilms tumors.
DISEASE:
Hypothyroidism, congenital, non-goitrous, 2 (CHNG2) [MIM:218700]: A disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue. Note=The disease is caused by mutations affecting the gene represented in this entry.
Similarity:
Contains 1 paired domain.
SWISS:
Q06710
Gene ID:
7849
Database links:Entrez Gene: 7849 Human
Entrez Gene: 18510 Mouse
Entrez Gene: 81819 Rat
Omim: 167415 Human
SwissProt: Q06710 Human
SwissProt: Q00288 Mouse
SwissProt: P51974 Rat
Unigene: 469728 Human
Unigene: 2533 Mouse
Unigene: 10392 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
同源结构域蛋白（Homeodomain Proteins）
PAX8属于转录抑制因子PAXs的一种。在胚胎发育和肿瘤生长中起关键作用。其突变和某些肿瘤的发病有关。
- 产品图片
Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-PAX8 Polyclonal Antibody, Unconjugated(bs-1201R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Cell:HT-29;
Concentration:1:100
Host/Isotype:Rabbit/IgG(blue line)
Flow cytometric analysis of Rabbit IgG isotype control on HT-29(blue) compared with primary antibody(Cat#: bs-1021R)(green) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .
Primary Antibody:Rabbit Anti-TLR4 antibody(bs-1021R, green line);Isotype Control Antibody: Rabbit IgG (orange line);Secondary Antibody: F(ab’)2 fragment goat anti-rabbit IgG-PE (white blue line)Positive control: Jurkat cells(blue line)
Protocol
1.Jurkat cells were washed twice with phosphate-buffered saline (PBS).2. An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 1 0 min at 37 °C.