Anti-Osterix【科瑞奥博】

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Anti-Osterix

货号： bs-1110R 基本售价： 380.0 元规格： 20ul

规格：20ul: 价格：380.00元

规格：50ul: 价格：780.00元

规格：100ul: 价格：1380.00元

规格：200ul: 价格：2200.00元

产品信息

产品编号: bs-1110R

英文名称: Osterix

中文名称: 成骨相关转录因子抗体

别名: Osterix; MGC126598; Osx; Sp 7; Sp7; Sp7 transcription factor; Transcription factor Sp7; Zinc finger protein osterix; SP7_HUMAN.

: Specific References  (13)     |     bs-1110R has been referenced in 13 publications.
[IF=6.13] Chen, Jin‐Ran, et al. "Diet derived phenolic acids regulate osteoblast and adipocyte lineage commitment and differentiation in young mice." Journal of Bone and Mineral Research (2013).  WB ;  Mouse.
PubMed:23832484
[IF=3.68] Dong, Weijie, et al. "Phenotypic characterization of craniofacial bone marrow stromal cells: unique properties of enhanced osteogenesis, cell recruitment, autophagy, and apoptosis resistance." Cell and Tissue Research (2014): 1-11.  WB ;  Rat.
PubMed:24927919
[IF=2.86] Kishi, Yuta, et al. "PARP Inhibitor PJ34 Suppresses Osteogenic Differentiation in Mouse Mesenchymal Stem Cells by Modulating BMP-2 Signaling Pathway."International Journal of Molecular Sciences 16.10 (2015): 24820-24838.  WB ;  Mouse.
PubMed:26492236
[IF=6.52] Agarwal, Shailesh, et al. "Analysis of bone‐cartilage‐stromal progenitor populations in trauma induced and genetic models of heterotopic ossification." STEM CELLS (2016).  IHC-P ;  Mouse.
PubMed:27068890
[IF=3.42] Tang, Zhurong, et al. "Comparison of ectopic bone formation process induced by four calcium phosphate ceramics in mice." Materials Science and Engineering: C (2016).  IHC-P ;  Mouse.
PubMed:27772699
[IF=0.00] Schlie-Wolter, S., et al. Osteogenic Differentiation of Human Adipose-Derived Stem Cells (hASC): Role of FAK and Ras-MAPK Signaling. (2016) J Stem Cell Regen Bio 2(2): 1- 19  WB ;  Human.
PubMed:0
[IF=5.23] Agarwal, Shailesh, et al. "Local and Circulating Endothelial Cells Undergo Endothelial to Mesenchymal Transition (EndMT) in Response to Musculoskeletal Injury." Scientific Reports 6 (2016): 32514.  WB ;  Mouse.
PubMed:27616463
[IF=5.30] Rajshankar, Dhaarmini, Yongqiang Wang, and Christopher A. McCulloch. "Osteogenesis requires FAK-dependent collagen synthesis by fibroblasts and osteoblasts." The FASEB Journal (2016): fj-201600645R.  WB ;  Mouse.
PubMed:27881487
[IF=1.56] Wang, Yilin, et al. "Synergistic effects of overexpression of BMP‑2 and TGF‑β3 on osteogenic differentiation of bone marrow mesenchymal stem cells." Molecular Medicine Reports (2016): 11.  WB ;  Rabbit.
PubMed:27878265
[IF=2.20] Ranganathan, Kavitha, et al. "The role of the adaptive immune system in burn-induced heterotopic ossification and mesenchymal cell osteogenic differentiation." Journal of Surgical Research 206.1 (2016): 53-61.  IF(IHC-P) ;  Mouse.
PubMed:27916375
[IF=5.23] Li, Shao, et al. "A Conditional Knockout Mouse Model Reveals a Critical Role of PKD1 in Osteoblast Differentiation and Bone Development." Scientific Reports 7 (2017): 40505.  WB ;  Mouse.
PubMed:28084409
[IF=2.33] Qian, Dong-Yang, et al. "Differential circRNA expression profiles during the BMP2-induced osteogenic differentiation of MC3T3-E1 cells." Biomedicine & Pharmacotherapy 90 (2017): 492-499.  WB ;  Mouse.
PubMed:28395271
[IF=0.57] Shi, Hua-Li, et al. "Effect of polygonimitin C on bone formation and resorption in human osteoblast-like MG63 cells." Tropical Journal of Pharmaceutical Research 16.7 (2017): 1595-1600.  WB ;  Human.
PubMed:0

规格价格: 50ul/780元购买 100ul/1380元购买 200ul/2200元购买大包装/询价

说明书: 50ul 100ul 200ul

研究领域: 细胞生物染色质和核信号信号转导干细胞转录调节因子

抗体来源: Rabbit

克隆类型: Polyclonal

交叉反应: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,

产品应用: WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量: 45kDa

细胞定位: 细胞核

性状: Lyophilized or Liquid

浓度: 1mg/ml

免疫原: KLH conjugated synthetic peptide derived from human Osterix:331-431/431

亚型: IgG

纯化方法: affinity purified by Protein A

储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed: PubMed

产品介绍

background:
This gene encodes a member of the Sp subfamily of Sp/XKLF transcription factors. Sp family proteins are sequence-specific DNA-binding proteins characterized by an amino-terminal trans-activation domain and three carboxy-terminal zinc finger motifs. This protein is a bone specific transcription factor and is required for osteoblast differentiation and bone formation.[provided by RefSeq, Jul 2010]

Function:
Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences (By similarity).

Subunit:
Interacts with NO66; the interaction is direct and inhibits transcription activator activity.

Subcellular Location:
Nucleus.

Tissue Specificity:
Osteoblast/chondrocyte specific.

DISEASE:
Osteogenesis imperfecta 12 (OI12) [MIM:613849]: A form of osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma. Disease severity ranges from very mild forms without fractures to intrauterine fractures and perinatal lethality. Extraskeletal manifestations, which affect a variable number of patients, are dentinogenesis imperfecta, hearing loss, and blue sclerae. OI12 is an autosomal recessive form characterized by recurrent fractures, mild bone deformations, generalized osteoporosis, delayed teeth eruption, no dentinogenesis imperfecta, normal hearing, and white sclerae. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the Sp1 C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.

SWISS:
Q8TDD2

Gene ID:
121340

Database links:

Entrez Gene: 121340 Human

Entrez Gene: 170574 Mouse

Entrez Gene: 300260 Rat

Omim: 606633 Human

SwissProt: Q8TDD2 Human

SwissProt: Q5RM08 Mouse

SwissProt: Q8VI67 Mouse

SwissProt: Q6IMK1 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Osterix成骨相关转录因子是一种具有锌指基序结构域的转录因子，在体内的表达对成骨细胞具有特异性。该蛋白只在发育的骨组织中特异性表达，是成骨细胞分化和骨形成过程中所必需的转录因子。骨髓基质干细胞分化为表达典型的成骨性标志基因的成骨细胞需要OSX的调控。

产品图片: Sample:Spinal cord (Mouse) Lysate at 40 ug
Primary: Anti-Osterix(bs-1110R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45kD
Observed band size: 50kD
Sample:
Embryo(Mouse) lysate at 30ug;
Embryo(Mouse) lysate at 30ug;
Primary: Anti-Osterix (bs-1110R) at 1:200 dilution;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 45kD
Observed band size : 45kD
Paraformaldehyde-fixed, paraffin embedded (Mouse embryos); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osterix) Polyclonal Antibody, Unconjugated (bs-1110R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.