产品中心
当前位置：首页>产品中心Anti-OCT2
货号： bs-1077R 基本售价： 780.0 元 规格： 50ul
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-1077R
- 英文名称
- OCT2
- 中文名称
- 阳离子转运蛋白2抗体
- 别 名
- organic cation transporter OCT2; POU domain, class 2, transcription factor 2; Octamer-binding transcription factor 2; Oct-2; OTF-2; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; POU2F2; NF A2; Oct 2; PO2F2_MOUSE; PO2F2_HUMAN.
- Specific References (1) | bs-1077R has been referenced in 1 publications.[IF=3.30] Xu C, Zhu L, Chan T, Lu X, Shen W, et al. (2015) The Altered Renal and Hepatic Expression of Solute Carrier Transporters (SLCs) in Type 1 Diabetic Mice. PLoS ONE 10(3): e0120760. doi:10.1371/journal.pone.0120760 WB ; Mouse.PubMed:25789863
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 细胞生物 免疫学 表观遗传学
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Human, Mouse, Rat,
- 产品应用
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 51kDa
- 细胞定位
- 细胞核 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from mouse OCT2:11-100/463
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Oct2 is a transcription factor that specifically binds to the octamer motif (5-ATTTGCAT-3). Oct2 regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. It also modulates transcription transactivation by NR3C1, AR and PGR. Oct2 is B cell specific, belongs to the POU transcription factor family class 2 subfamily and contains 1 homeobox domain.
Function:
Transcription factor that specifically binds to the octamer motif (5-ATTTGCAT-3). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5 activates the U2 small nuclear RNA (snRNA) promoter.
Subunit:
Interacts with NR3C1, AR and PGR.
Subcellular Location:
Cytoplasm. Nuclear
Tissue Specificity:
Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line Molt-4, but not in the T-cell line BW 5147.
Similarity:
Belongs to the POU transcription factor family. Class-2 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
SWISS:
Q00196
Gene ID:
18987
Database links:Entrez Gene: 5452 Human
Entrez Gene: 18987 Mouse
Entrez Gene: 117058 Rat
Omim: 164176 Human
SwissProt: P09086 Human
SwissProt: Q00196 Mouse
Unigene: 147381 Human
Unigene: 76683 Mouse
Unigene: 155998 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
同源结构域蛋白（Homeodomain Proteins）
OCT-2是B淋巴细胞转录因子，以免疫球蛋白启动子为目标，于生发中心形成和B细胞分化成浆细胞中起作用。
- 产品图片
Sample:
A549 Cell lysate at 30 ug;
Primary: Anti-OCT2 (bs-1077R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution;
Predicted band size : 51 kD
Observed band size : 51 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-OCT2 Polyclonal Antibody, Unconjugated(bs-1077R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-OCT2 Polyclonal Antibody, Unconjugated(bs-1077R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining