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货号： bs-0008R 基本售价： 380.0 元 规格： 20ul
- 规格：20ul
- 价格：380.00元
- 规格：50ul
- 价格：780.00元
- 规格：100ul
- 价格：1380.00元
- 规格：200ul
- 价格：2200.00元
产品信息
- 产品编号
- bs-0008R
- 英文名称
- Nestin
- 中文名称
- 巢蛋白/神经上皮干细胞蛋白抗体
- 别 名
- ESTM 46; FLJ 21841; FLJ21841; Intermediate filament protein; Nbla00170; Nes; NEST_RAT; Nestin.
- Specific References (3) | bs-0008R has been referenced in 3 publications.[IF=3.17] Liu, Yang, et al. "Amelioration of Stroke-Induced Neurological Deficiency by Lyophilized Powder of Catapol and Puerarin." International Journal of Biological Sciences 10.4 (2014): 448-456. IHC-P ; Mouse.PubMed:24719562[IF=1.64] Shan, Tengfei, et al. "Lithium chloride promotes the odontoblast differentiation of hair follicle neural crest cells by activating Wnt/β‐catenin signaling." Cell Biology International (2014). IF(ICC) ; Mouse.PubMed:25044369[IF=0.97] Kawashima, Satoki, Koichi SHINKAI, and Masaya SUZUKI. "Effect of an experimental adhesive resin containing multi-ion releasing fillers on direct pulp-capping." Dental Materials Journal 35.3 (2016): 479-489. IHC-P ; Rat.PubMed:27252005
- 规格价格
- 50ul/780元购买 100ul/1380元购买 200ul/2200元购买 大包装/询价
- 说 明 书
- 50ul 100ul 200ul
- 研究领域
- 肿瘤 心血管 发育生物学 神经生物学 干细胞 细胞类型标志物 肿瘤细胞生物标志物
- 抗体来源
- Rabbit
- 克隆类型
- Polyclonal
- 交叉反应
- Mouse, Rat,
- 产品应用
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
- 分 子 量
- 209kDa
- 细胞定位
- 细胞浆
- 性 状
- Lyophilized or Liquid
- 浓 度
- 1mg/ml
- 免 疫 原
- KLH conjugated synthetic peptide derived from rat Nestin:681-750/1893
- 亚 型
- IgG
- 纯化方法
- affinity purified by Protein A
- 储 存 液
- 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 保存条件
- Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
- PubMed
- PubMed
- 产品介绍
- background:
Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tumor endothelial cells in the mammalian CNS. Furthermore, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumor.
Function:
Required for brain and eye development (By similarity). Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Subunit:
Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, this protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3.
Subcellular Location:
cytoplasm
Tissue Specificity:
CNS stem cells.
Post-translational modifications:
Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized.
Similarity:
Belongs to the intermediate filament family.
SWISS:
P21263
Gene ID:
25491
Database links:Entrez Gene: 10763 Human
Entrez Gene: 18008 Mouse
Entrez Gene: 25491 Rat
Omim: 600915 Human
SwissProt: P48681 Human
SwissProt: Q6P5H2 Mouse
SwissProt: P21263 Rat
Unigene: 527971 Human
Unigene: 331129 Mouse
Unigene: 9701 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
神经干细胞标志物
在动物体早期发育过程中，中枢神经系统发育尤为重要 。Nestin是一种中等纤维蛋白，它在哺乳动物神经前体细胞中表达较高，目前已被广泛用于神经前体细胞的重要标志分子。研究Nestin的表达调控规律，将有助于人们认识神经前体细胞的生物特性。此抗体还表达中枢神经系统干细胞及神经上皮细胞。
- 产品图片
Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nestin Polyclonal Antibody, Unconjugated(bs-0008R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
Cell: Neuro-2a
Concentration:1:100
Host/Isotype:Rabbit/IgG
Flow cytometric analysis of Rabbit IgG isotype control on Neuro-2a(blue) compared with primary antibody(Cat#: bs-0008R)(green) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .