Anti-MMP-3【科瑞奥博】

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Anti-MMP-3

货号： bs-0413R 基本售价： 380.0 元规格： 20ul

规格：20ul: 价格：380.00元

规格：50ul: 价格：780.00元

规格：100ul: 价格：1380.00元

规格：200ul: 价格：2200.00元

产品信息

产品编号: bs-0413R

英文名称: MMP-3

中文名称: 基质金属蛋白酶3抗体

别名: matrix metalloproteinase-3; Transin-1; SL-1; SL 1; SL1; Stromelysin-1 precursor; MMP3 Stromelysin-1; EMS-2; Matrix metalloproteinase 3; Matrix metalloproteinase-3; Matrix metalloproteinase 3 preproprotein; MGC126102; MGC126103; MGC126104; MMP 3; MMP3; Progelatinase; Proteoglycanase;STMY; STMY1; STR1; Stromelisin 1; Stromelysin 1 progelatinase; Transin 1; MMP3_HUMAN.

: Specific References  (8)     |     bs-0413R has been referenced in 8 publications.
[IF=3.82] Luo, Yang, et al. "Alendronate Retards the Progression of Lumbar Intervertebral Disc Degeneration in Ovariectomized Rats." Bone (2013).  IHC-P ;  Rat.
PubMed:23500174
[IF=2.47] Luo, Yang, et al. "The inhibitory effect of salmon calcitonin on intervertebral disc degeneration in an ovariectomized rat model." European Spine Journal (2014): 1-11.  IHC-P ;  Rat.
PubMed:25304649
[IF=0.98] Koch, Holger, et al. "Tissue engineering of ureteral grafts: Preparation of biocompatible crosslinked ureteral scaffolds of porcine origin." Name: Frontiers in Bioengineering and Biotechnology 3 (2015): 89.  IHC-P ;  Pig.
PubMed:26157796
[IF=1.43] Wang, Xiao-yan, et al. "AMD3100 attenuates MMP-3 and MMP-9 expressions and prevents cartilage degradation in a monosodium iodoacetate-induced rat model of temporomandibular osteoarthritis." Journal of Oral and Maxillofacial Surgery (2016).  IHC-P ;  Rat.
PubMed:26851314
[IF=2.43] Wei, Fuxin, et al. "Pingyangmycin-induced in vivo lumbar disc degeneration model of rhesus monkeys." Spine 40.4 (2015): E199-E210.  IHC-P ;
PubMed:25679953
[IF=2.14] Lu, Minling, et al. "The effects of mycotoxins and selenium deficiency on tissue-engineered cartilage." Cells Tissues Organs 196.3 (2012): 241-250.  IHC-P ;  Rabbit.
PubMed:22538829
[IF=1.80] Han, Jing, et al. "Long-Term Selenium-Deficient Diet Induces Liver Damage by Altering Hepatocyte Ultrastructure and MMP1/3 and TIMP1/3 Expression in Growing Rats." Biological Trace Element Research (2016): 1-9.  IHC-P ;  Rat.
PubMed:27339256
[IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082.  WB ;  Rat.
PubMed:27665780

规格价格: 50ul/780元购买 100ul/1380元购买 200ul/2200元购买大包装/询价

说明书: 50ul 100ul 200ul

研究领域: 肿瘤

抗体来源: Rabbit

克隆类型: Polyclonal

交叉反应: Human, Mouse, Rat, Dog, Pig, Horse, Sheep, Guinea Pig,

产品应用: WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分子量: 42/52kDa

细胞定位: 细胞外基质分泌型蛋白

性状: Lyophilized or Liquid

浓度: 1mg/ml

免疫原: KLH conjugated synthetic peptide derived from human MMP3:401-477/477

亚型: IgG

纯化方法: affinity purified by Protein A

储存液: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed: PubMed

产品介绍

background:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008].

Function:
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Subcellular Location:
Secreted, extracellular space, extracellular matrix (Probable).

Similarity:
Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.

SWISS:
P08254

Gene ID:
4314

Database links:

Entrez Gene: 4314 Human

Entrez Gene: 17392 Mouse

Entrez Gene: 171045 Rat

Omim: 185250 Human

SwissProt: P08254 Human

SwissProt: P28862 Mouse

SwissProt: P03957 Rat

Unigene: 375129 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

合成与降解（Synthesis and Degradation）

基质金属蛋白酶(matrix metalloproteinases, MMPs)是一族依赖锌离子而降解各种细胞外基质的蛋白酶，亦称IV型胶原酶或称明胶酶A，其主要功能为降解IV型胶原，因而它在肿瘤细胞突破基底膜屏障和浸润转移中起重要作用。
MMP3目前主要用于各种恶性肿瘤(如乳腺癌、胃肠道癌、卵巢癌、膀胱癌等)中的基底膜检测与肿瘤转移浸润的研究。

产品图片: Sample:
HepG2(Human) Cell Lysate at 30 ug
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-MMP-3 (bs-0413R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42/52 kD
Observed band size: 52 kD
Sample:Lymph node (Mouse) Lysate at 30 ug
Primary: Anti- MMP-3 (bs-0413R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42/52kD
Observed band size: 52kD
Tissue/cell: human gastric mucosa; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MMP-3 Polyclonal Antibody, Unconjugated(bs-0413R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining